30,808 results

1. p70rg 15

   Plasmid

   #17831

   Depositor

   Jay Keasling

   Article

   Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

   Insert

   E. coli optimized red flourescent protein (RFPec), GFPuv
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

2. R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

   Plasmid

   #159543

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   G13D

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmVirD2

   Plasmid

   #90314

   Purpose

   mVirD2 expression plasmid

   Depositor

   Bjorn Hogberg

   Article

   Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

   Insert

   A. tumefaciens VirD2 from aa 1 to 204
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7/T5 IPTG inducible

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLlR5

   Plasmid

   #149481

   Purpose

   Broad-Host range expression vector, RSF1010 origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LacI-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLlacO-1

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXA4 (murine) HA-tag pET

   Plasmid

   #8489

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXA4 (Hoxa4 Mouse)
   

   Tags

   HA and T7

   Expression

   Bacterial

   Mutation

   Thr-2 changed to Ala-2 to form better ribosomal b…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pETM6-P.accABCD

   Plasmid

   #66866

   Purpose

   Encodes accA, accB, accC, accD in Pseudo-operon configuration.

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   accA
   accB
   accC
   accD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. J-HARS-4

   Plasmid

   #166529

   Purpose

   scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. L-SARS-7

   Plasmid

   #166527

   Purpose

   scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Promoter

   LacZ

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTET-IUPi

   Plasmid

   #122019

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pTET

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX4T1-TEV-HsFbxo7-129-398/Skp1

    Plasmid

    #202592

    Purpose

    Bacterial expression of HsFbxo7-129-398 and HsSkp1

    Depositor

    Brenda Schulman

    Article

    Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

    Inserts

    Fbxo7 (FBXO7 Human)
    Skp1 (SKP1 Human)
    

    Tags

    GST, TEV

    Expression

    Bacterial

    Mutation

    aa 129-398 only

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTP443

    Plasmid

    #216361

    Purpose

    xNup98-Nb1t (+1Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup98-Nb1t (+1Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLD-CAG-DYSF-CFL

    Plasmid

    #131457

    Purpose

    Encodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    DYSF and Follistatin (DYSF, FST Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    R15C (in DYSF) and Q2579H (in CFL)

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAT2

    Plasmid

    #112588

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-GST

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28 His10-FcRɣ

    Plasmid

    #113021

    Purpose

    for purification of His10-FcRɣ intracellular domain

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    His10-FcRɣ intracellular domain
    

    Expression

    Bacterial

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIB167

    Plasmid

    #90190

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSG13-pBAD-TetRssrA

    Plasmid

    #102449

    Purpose

    Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    TetR (tetR )
    

    Tags

    ssrA

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAL-pgi_NT

    Plasmid

    #74067

    Purpose

    pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pgi (NT)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046758

    Plasmid

    #145670

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pETM30-C9ORF78_F8A

    Plasmid

    #193369

    Purpose

    Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

    Depositor

    Markus Wahl

    Article

    Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

    Insert

    C9ORF78_F8A (C9orf78 Human)
    

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    F8A, the mutation is located in the binding inter…

    Promoter

    T7

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMJ915v2+Nterm Spe1 +Cterm Age1 site

    Plasmid

    #88914

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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