30,736 results

1. MDO2-c005

   Plasmid

   #173106

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD2 (MACROD2 Human)
   

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   K7-D243

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-EsaR-V220A

   Plasmid

   #47645

   Purpose

   pAC-EsaR with a c659t mutation in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-V220A
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed Valine 220 to Alanine

   Promoter

   Plac

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pMT-MCS-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80058

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1-p19-T7-PLK1

   Plasmid

   #46312

   Depositor

   Judy Lieberman

   Article

   Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

   Inserts

   p19
   PLK1 DNA-1
   linker
   PLK1 DNA-2
   

   Use

   Pro-sirna

   Tags

   6XHis and GST

   Expression

   Bacterial

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pNIC28-Bsa4-ICL1

   Plasmid

   #105822

   Purpose

   Expression of Mycobacterium tuberculosis icl1 recombinant protein

   Depositor

   Ivanhoe Leung

   Article

   Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

   Insert

   icl1
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-tTALE-EmGFP-15b

   Plasmid

   #138280

   Purpose

   Express tTALE-EmGFP

   Depositor

   Kyubong Jo

   Article

   Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

   Insert

   7-mer tTALE-EmGFP
   

   Use

   TALEN

   Tags

   6x His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBR5pemIK-GW

   Plasmid

   #126556

   Purpose

   Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatible

   Depositor

   Lindsey Burbank, Drake Stenger

   Article

   Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_SERPINA5_Serpin

   Plasmid

   #110034

   Purpose

   Protein expression and purification of SERPINA5_Serpin

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   SERPINA5_Serpin (SERPINA5 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC165

   Plasmid

   #65949

   Purpose

   P1 activator plasmid for two-strain system

   Depositor

   Matthew Bennett

   Article

   Chen et al Science. 2015 Aug 28;349(6251):986-9. do

   Insert

   RhlI (rhlI )
   

   Tags

   LAA tagged

   Expression

   Bacterial

   Promoter

   pLLac*

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TBXTA-c027

    Plasmid

    #139764

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, Full-length, G177D (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-GST-TEV

    Expression

    Bacterial

    Mutation

    G177D; contains only amino acids S2- M435

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid

    #101718

    Purpose

    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEcCas6

    Plasmid

    #170091

    Purpose

    expresses E. coli type I-E Cas6

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    E. coli type I-E Cas6
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available Since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pBXC3H

    Plasmid

    #47068

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB1-2ndVal mGFPmut2

    Plasmid

    #104033

    Purpose

    Plasmid encoding 2ndVal mGFPmut2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    2ndVal mGFPmut2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    mGFPmut2 with valine inserted in 2nd position

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET32a-hHBEGF

    Plasmid

    #199234

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Proheparin-binding EGF-like growth factor (HBEGF Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2-cryR;lmo2P1Egfp

    Plasmid

    #90162

    Purpose

    contains lmo2 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    lmo2P1Egfp (lmo2 Zebrafish)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-M-4A6

    Plasmid

    #73434

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4A6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T-1-Skp2 WT

    Plasmid

    #81117

    Purpose

    bacterial expression plasmid of Skp2

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (SKP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

20. NRDP1

    Plasmid

    #25620

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NRDP1 (RNF41 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains USP8 interaction Domain

    Available Since

    Aug. 31, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits