30,736 results

1. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-prk:nptII

   Plasmid

   #239580

   Purpose

   Selection plasmid for Rubisco directed evolution

   Depositor

   Matthew D. Shoulders

   Insert

   PRK-NPTII
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF_AtRaf1/Raf2/RbcX2/BSD2/RbcX1

   Plasmid

   #229510

   Purpose

   Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
   Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
   RbcX2 (RbcX2 Mustard Weed)
   BSD2
   RbcX1
   

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30-HyPer7

   Plasmid

   #141071

   Purpose

   Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Hisx6

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18

   Plasmid

   #50004

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1E1D1

   Plasmid

   #161605

   Purpose

   Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E1D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pET12a-PAPI

   Plasmid

   #19961

   Depositor

   Jonathan Tait

   Article

   Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

   Insert

   Annexin V (ANXA5 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7RNAP Cyslight

   Plasmid

   #205077

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight (T7p07 Bacteriophage T7)
   

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Tags

    6xHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRE112

    Plasmid

    #43828

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. SPYtag-GFPnanobody

    Plasmid

    #214837

    Purpose

    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    SPYtag-GFPnanobody
    

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYC01

    Plasmid

    #224586

    Purpose

    IPTG-inducible phage φX174 lysis gene E

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    phage φX174 lysis gene E (phiX174p08 )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PA1lacO-1

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJJS002

    Plasmid

    #188325

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    Linker::GFP::mIAA7
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. COC053

    Plasmid

    #209131

    Purpose

    Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

    Depositor

    Christopher A. O'Callaghan

    Article

    O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

    Insert

    Enzyme BirA (birA E. coli)
    

    Expression

    Bacterial

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid

    #85165

    Purpose

    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

    Depositor

    Robin Dowell

    Article

    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

    Insert

    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    

    Tags

    AmilCP and RTX

    Expression

    Bacterial

    Promoter

    J23100 promoter

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. spNW80

    Plasmid

    #173487

    Purpose

    Expresses spNW80 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW80
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBR322-PVCpnf17-22

    Plasmid

    #198272

    Purpose

    Unmodified pPayload; contains WT PVC payloads/regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf17-22
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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