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pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

Plasmid

#29723

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mO…

Expression

Bacterial

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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monomer-HD

Plasmid

#27968

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-HD

Expression

Bacterial

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTC-28S15-0X

Plasmid

#20316

Depositor

Dirk Schnappinger

Publication

Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

Insert

Psmyc-tetR#28

Expression

Bacterial

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET3a-Yuh1

Plasmid

#18895

Depositor

Cecile Pickart

Publication

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)

Expression

Bacterial

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pB1H2wL-Prd

Plasmid

#18040

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

Paired (prd Fly)

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-mutOdd

Plasmid

#18044

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

mutant Odd Skipped

Expression

Bacterial

Mutation

A mutant version of the zinc finger protein Odd S…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR FOXP3

Plasmid

#16363

Depositor

Anjana Rao

Publication

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Tags

Myc

Expression

Bacterial

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Pdx1-RFP puro (DM#235)

Plasmid

#15085

Depositor

Douglas Melton

Publication

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Tags

RFP

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
Dlx5

Plasmid

#15538

Depositor

John Rubenstein

Publication

Zerucha et al J Neurosci. 2000 Jan 15. 20(2):709-21.

Insert

Dlx5 (Dlx5 Mouse)

Expression

Bacterial

Available since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pGEX-NEMO

Plasmid

#11965

Depositor

Jon Ashwell

Publication

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Tags

GST

Expression

Bacterial

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

Plasmid

#212829

Purpose

Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

Depositor

Wolfgang Enard

Publication

Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

Insert

ZIM3-KRAB-dCas9-P2A-mCherry

Tags

HA-tag and ZIM3-KRAB

Expression

Bacterial and Mammalian

Promoter

CAG, TetOn

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-Benzonase

Plasmid

#218192

Purpose

Bacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001

Depositor

Cheryl Arrowsmith

Publication

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

MBP

Expression

Bacterial

Available since

April 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Publication

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD-bARGSer-AxeTxe

Plasmid

#192473

Purpose

Second-generation bacterial acoustic reporter gene derived from Serratia

Depositor

Mikhail Shapiro

Publication

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Inserts

bARGSer
AxeTxe

Expression

Bacterial

Mutation

the gene Ser39006_001280 was deleted from the clu…

Promoter

Native promoter from Enterococcus faecium and pBAD

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHyo245

Plasmid

#173147

Purpose

Expresses pheS_A294G by dual T7 promoter in e.coli

Depositor

Seokhee Kim

Publication

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pheS_A294G

Expression

Bacterial

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#167547

Purpose

Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Depositor

Howard Hang

Publication

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Inserts

chloramphenicol acetyl transferase
pUC19 origin of replication

Use

CRISPR

Expression

Bacterial

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Publication

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046828

Plasmid

#145742

Purpose

Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pHAGE2-TetOminiCMV-OSKM

Plasmid

#136541

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

Depositor

Hans Schöler

Publication

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDNA2.0 6H-FLAG-TEV-KRAS G12C

Plasmid

#111848

Purpose

Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS G12C (KRAS Human)

Tags

6xHis-tag, FLAG-tag, and TEV protease cleavage se…

Expression

Bacterial

Mutation

Changed Glycine 12 to Cysteine

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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