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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Publication

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#167547

Purpose

Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Depositor

Howard Hang

Publication

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Inserts

chloramphenicol acetyl transferase
pUC19 origin of replication

Use

CRISPR

Expression

Bacterial

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-Bxb1-His

Plasmid

#123132

Purpose

Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

Depositor

David Liu

Publication

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Bxb1 integrase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a_TIA1

Plasmid

#106095

Purpose

bacterial protein expression of TIA1

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

6xHIs-thrombin and T7

Expression

Bacterial

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Publication

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pUltra-sY

Plasmid

#82417

Purpose

Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

Depositor

Chang Liu

Publication

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Methanococcus jannaschii aaRS for sulfotyrosine

Expression

Bacterial

Mutation

Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

Promoter

tacI

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP3

Plasmid

#79875

Purpose

Bacillus subtilis sgRNA expression vector; integrates into thrC

Depositor

Carol Gross, Jason Peters, Stanley Qi

Publication

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Publication

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUCP20T-eyfp

Plasmid

#78466

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Publication

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eYFP

Expression

Bacterial

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pComb8

Plasmid

#63889

Purpose

For phage display of antibody libraries by fusion to modified geneVIII.

Depositor

Carlos Barbas

Publication

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneVIII

Expression

Bacterial

Promoter

lacZ

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Publication

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCM433kanT

Plasmid

#61262

Purpose

Sucrose counterselection (sacB) vector for unmarked allelic exchange.

Depositor

Mary Lidstrom

Publication

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Type

Empty backbone

Use

Synthetic Biology; Sucrose counterselection

Expression

Bacterial

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-His-hAos1

Plasmid

#53135

Purpose

E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

Depositor

Frauke Melchior

Publication

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Aos1 (SAE1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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EBFP2-pBAD

Plasmid

#54542

Purpose

Localization: Protein Expression Vector , Excitation: 383, Emission: 448

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and EBFP2

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Publication

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET22b-CPDSalI

Plasmid

#38251

Depositor

Matthew Bogyo, Aimee Shen

Publication

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

Plasmid

#29723

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mO…

Expression

Bacterial

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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monomer-HD

Plasmid

#27968

Depositor

Feng Zhang

Publication

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-monomer-HD

Expression

Bacterial

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28GST-LIC

Plasmid

#26101

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6XHis, GST, and Thrombin CLeavage Site

Expression

Bacterial

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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