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pEGFP_PCNA_IRES_puro2b

Plasmid

#21048

Depositor

Daniel Gerlich

Publication

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

hPCNA

Tags

GFP

Expression

Bacterial and Mammalian

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Publication

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Expression

Bacterial

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB1H2wL-Prd

Plasmid

#18040

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

Paired (prd Fly)

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pTn Donor

Plasmid

#236211

Purpose

Arabinose-inducible Tn5 InducTn-seq plasmid

Depositor

Matthew Waldor

Publication

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

None

Expression

Bacterial

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5Knob

Plasmid

#198289

Purpose

Produces PVCs retargeted with knob domain from hAd5 virus

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5Knob

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Publication

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Publication

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#167547

Purpose

Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Depositor

Howard Hang

Publication

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Inserts

chloramphenicol acetyl transferase
pUC19 origin of replication

Use

CRISPR

Expression

Bacterial

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETduet+p300HAT

Plasmid

#157793

Purpose

Expression of p300 HAT domain

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

p300HAT (EP300 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEvol-MjaYRS

Plasmid

#153557

Purpose

Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

Depositor

Huiwang Ai

Publication

Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

Inserts

MJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter

Expression

Bacterial

Promoter

glnS and pBAD

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a_TIA1

Plasmid

#106095

Purpose

bacterial protein expression of TIA1

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

6xHIs-thrombin and T7

Expression

Bacterial

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Publication

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pUltra-sY

Plasmid

#82417

Purpose

Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

Depositor

Chang Liu

Publication

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Methanococcus jannaschii aaRS for sulfotyrosine

Expression

Bacterial

Mutation

Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

Promoter

tacI

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP3

Plasmid

#79875

Purpose

Bacillus subtilis sgRNA expression vector; integrates into thrC

Depositor

Carol Gross, Jason Peters, Stanley Qi

Publication

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pComb8

Plasmid

#63889

Purpose

For phage display of antibody libraries by fusion to modified geneVIII.

Depositor

Carlos Barbas

Publication

Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

Type

Empty backbone

Use

Phage display

Tags

geneVIII

Expression

Bacterial

Promoter

lacZ

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Publication

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCM433kanT

Plasmid

#61262

Purpose

Sucrose counterselection (sacB) vector for unmarked allelic exchange.

Depositor

Mary Lidstrom

Publication

Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

Type

Empty backbone

Use

Synthetic Biology; Sucrose counterselection

Expression

Bacterial

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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EBFP2-pBAD

Plasmid

#54542

Purpose

Localization: Protein Expression Vector , Excitation: 383, Emission: 448

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and EBFP2

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Publication

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article