32,295 results
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Plasmid#212917PurposeEncodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA1 (AGA1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD083
Plasmid#212920PurposeEncodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSED1 (SED1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD003
Plasmid#212900PurposeEncodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertECM14 (ECM14 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD081
Plasmid#212918PurposeEncodes the HA-tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT
Plasmid#192959PurposeFor expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studiesDepositorInsertsptse5-CT
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM236 Ubc4
Plasmid#226361PurposeExpression of yeast Ubc4DepositorAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-1UAG
Plasmid#82500PurposeExpresses GFP with 1 UAG codon at the amino acid position 3DepositorInsertGreen Fluorescent Protein with 1 UAG codon
ExpressionBacterialMutationAdded an UAG codon at the amino acid position 3PromoterAraBADAvailable SinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FMRP_P451S
Plasmid#198693PurposeExpression of human FMRP protein in E. coliDepositorInsertFMR1 (FMR1 Human)
TagsMBPExpressionBacterialMutationchanged proline 451 to serinePromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-Ubiquitin
Plasmid#223236PurposeBacterial expression construct for GST-Tagged Ubiquitin production and purificationDepositorInsertUbiquitin
ExpressionBacterialPromotertacAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX iLOV
Plasmid#26587DepositorInsertiLOV
TagsGSTExpressionBacterialAvailable SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTKRED
Plasmid#41062DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorAvailable SinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Matr3 delRRM2
Plasmid#32882DepositorInsertMATR3 Matrin3 (MATR3 Human)
TagsFLAGExpressionBacterial and MammalianMutationdeleted amino acids 497-566PromoterCMVAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-CTwinStrep
Plasmid#45941Purposeuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTagsPelB and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDM028
Plasmid#216809PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only