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Bacterial Expression

30,738 results

Showing: 1581 - 1600 of 30738 results

  1. pMSP3545

    Plasmid
    #46888
    Purpose
    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression
    Depositor
    Gary Dunny
    Article
    Bryan et al Plasmid. 2000 Sep;44(2):183-90.
    Type
    Empty backbone
    Use
    Nisin-inducible expression; gram-positive bacteri…
    Expression
    Bacterial
    Promoter
    PnisA
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    46888-plasmid-map-sequence-id-347882

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  3. pNBU2_repA1

    Plasmid
    #211910
    Purpose
    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211910-plasmid-map-sequence-id-421620

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  5. pCDF-5xT7-TtCsm

    Plasmid
    #128572
    Purpose
    For expression of T. thermophilus Csm in E. coli (Csm1-5)
    Depositor
    Jennifer Doudna
    Article
    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:
    Inserts
    Csm1
    Csm2, Csm3, Csm4, Csm5
    Tags
    HRV 3C protease cleavage site and 10xHis tag on C…
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli and Cod…
    Promoter
    T7 promoter, lac operator
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128572-plasmid-map-sequence-id-252577

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  6. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  8. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  9. pNR291

    Plasmid
    #79555
    Purpose
    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    GFPmut3b
    mTagBFP
    mRFP
    Expression
    Bacterial
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79555-plasmid-map-sequence-id-148766

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  15. pGEX6P1-3xMBT

    Plasmid
    #46987
    Purpose
    Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli
    Depositor
    Or Gozani
    Article
    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:
    Insert
    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    Tags
    GST and PreScission cleavage site
    Expression
    Bacterial
    Mutation
    contains amino acids 190–530 of accession NP_0562…
    Promoter
    tac
    Available Since
    Sept. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    46987-plasmid-map-sequence-id-66939

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  16. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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Showing: 1581 - 1600 of 30738 results