29,690 results

1. pCFJ1320

   Plasmid

   #84829

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD56

   Plasmid

   #119883

   Purpose

   Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pBAD_EYFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRDM1

   Plasmid

   #25249

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PRDM1_04 (PRDM1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pCold-TF-hRubicon

   Plasmid

   #64147

   Purpose

   For protein expression in E. coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pReporter_12

   Plasmid

   #60572

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. p15A-OptoCre-knt-P**-R

   Plasmid

   #189851

   Purpose

   Cre-inducible activation of knt for kanamycin resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P**-loxP-TT-loxP-R-knt
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P** (modified T7 phage gene 10)

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p15A-OptoCre-cat-P-R

   Plasmid

   #189853

   Purpose

   Cre-inducible activation of cat for chloramphenicol resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P-loxP-TT-loxP-R-cat
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P (T7 phage gene 10)

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAT204

   Plasmid

   #134881

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC009.106

   Plasmid

   #171142

   Purpose

   Expression of J106 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J106 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAP114

    Plasmid

    #120799

    Purpose

    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Insert

    Pxyl::mCherryOpt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

    Promoter

    Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE131

    Plasmid

    #118485

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-nCas9n

    Plasmid

    #46757

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAWP78-PVCpnf_pvc13-CD11bDARPin

    Plasmid

    #198295

    Purpose

    Produces PVCs retargeted with anti-CD11b DARPin F7

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-CD11bDARPin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PfICH pET15b

    Plasmid

    #60684

    Purpose

    Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

    Insert

    inhA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pAIP4_3KB5

    Plasmid

    #234225

    Purpose

    Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3KB5 (TRIM72 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. AfNitB_pET29b

    Plasmid

    #215401

    Purpose

    Expression construct for AfNitB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    AfNitB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPPC005

    Plasmid

    #171140

    Purpose

    For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    XylS-Pm
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-EPN51 (R40A)

    Plasmid

    #80109

    Purpose

    Late1-O3-33-myc-PH(R40A)

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-51 (R40A)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only