30,535 results

1. pSW002-Pc-mTurquoise2

   Plasmid

   #108235

   Purpose

   Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   Pc promoter - mTurquoise2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for experession in P. fluorescens

   Promoter

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pACYC-T7

   Plasmid

   #41187

   Depositor

   Dan Bolon

   Article

   Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

   Insert

   S. cerevisiae Hsp82
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET Flag TEV LIC cloning vector (2L-T)

   Plasmid

   #29715

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   FLAG-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-29b(+)_His-CBD-SpEndoH

   Plasmid

   #218196

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

   Depositor

   Florence Pojer

   Insert

   EndoH
   

   Use

   Tags

   tufA-His-CBD

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-313

   Promoter

   T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSFDuet-sumo-h-cGAS

   Plasmid

   #127161

   Purpose

   expression of human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTR47m4-GFP

   Plasmid

   #102436

   Purpose

   Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

   Depositor

   Gregory Stephanopoulos

   Article

   Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

   Inserts

   Formaldehyde repressor protein (frmR)
   Superfolder GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pATZ-Myc

   Plasmid

   #177718

   Purpose

   ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   Myc

   Expression

   Bacterial and Mammalian

   Mutation

   E342K (ATZ mutant)

   Promoter

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV/SB11

   Plasmid

   #26552

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   Sleeping Beauty
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Methionine 243 to Glutamine Threonine 136 to Arg…

   Promoter

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSUMO2

    Plasmid

    #52259

    Purpose

    Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-2 (SUMO2 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSUMO3

    Plasmid

    #52260

    Purpose

    Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-3 (SUMO3 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Pet28a_Human_H4

    Plasmid

    #42633

    Depositor

    Joe Landry

    Insert

    Human H4 (H4C1 Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ecALDH

    Plasmid

    #67921

    Purpose

    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Insert

    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTSara

    Plasmid

    #60720

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T7 RNAP split between positions 179-180 and wt T7…

    Promoter

    PBAD

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC18T-mini-Tn7T-Gm-Pc-tdTomato

    Plasmid

    #199250

    Purpose

    Fluorescent labelling of bacterial cells with tdTomato

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized tdTomato gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-Neq2X7

    Plasmid

    #182366

    Purpose

    Expresses Neq2X7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X7 DNA polymerase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLPT107

    Plasmid

    #85525

    Purpose

    Repressilator with triple reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    mKate2
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge