32,870 results
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Plasmid#114070PurposeT7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tagDepositorInsertbacteria codon optimized LbCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
T777T
Plasmid#113082PurposeEnhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Plac-dCas9 (Plac)
Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-lvaA
Plasmid#106397PurposeGuide RNA plasmid targeting lvaA on BBR1-UP originDepositorInsertsgRNA towards lvaA in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AsCpf1-2NLS
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_WT
Plasmid#98653PurposeExpresses MBP-tagged FUS AA 1-163DepositorAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
Tags10xHis-MBP-TEVExpressionBacterialAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW005-2
Plasmid#85611PurposeEmpty gRNA delivery vector for Bacillus subtilisDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCN-mBFP
Plasmid#63847PurposeEncodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.DepositorInsertmBFP
ExpressionBacterialMutationPromoter and RBS was swapped. His-tag was removed…Available SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Km
Plasmid#45938Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJ251-GERC
Plasmid#47441PurposeExpresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.DepositorInsertGene Expression Reporter Construct
ExpressionBacterialAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCambia1300-PacI
Plasmid#44183DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCsy4
Plasmid#44252DepositorInsertcsy4 gene
UseSynthetic BiologyExpressionBacterialPromoterpLtetO-1Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange2
Plasmid#34957DepositorInsertPSmOrange2
ExpressionBacterialMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …Available SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBG100-TCPTP
Plasmid#33365DepositorAvailable SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGex-Cav1
Plasmid#33364DepositorAvailable SinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
ExpressionBacterialAvailable SinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only