30,535 results

1. pGEM4Z-EGFP

   Plasmid

   #183475

   Purpose

   This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAIP1

   Plasmid

   #233685

   Purpose

   Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

   Depositor

   Christopher Bahl

   Insert

   Ulp1-like SUMO protease
   

   Use

   Tags

   CAHS-107838 and HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pnEA-vH_His-TEV-SEPT2_SEPT6

   Plasmid

   #174497

   Purpose

   bacterial co-expression of human SEPT2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBolux

   Plasmid

   #188109

   Purpose

   Encodes a promoter-less Bacteroides-optimized lux cassette

   Depositor

   Guy Townsend

   Article

   Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQE30-His-AlaRS

   Plasmid

   #124103

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Alanyl-tRNA ligase (alaS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLG001 (empty vector)

   Plasmid

   #157879

   Purpose

   Empty vector backbone for defense systems

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   None
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MP1

   Plasmid

   #69627

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 umuD' umuC recA730
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid

    #228663

    Purpose

    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Zika virus portion of NS2B protease coexpressed with NS3 protease
    

    Use

    Tags

    His-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pET28:LanIII

    Plasmid

    #225075

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIII)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28:LanIII-43

    Plasmid

    #225077

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIII-43)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28:LanII

    Plasmid

    #225066

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDM068

    Plasmid

    #216811

    Purpose

    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Type

    Empty backbone

    Use

    CRISPR; Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDM053

    Plasmid

    #216815

    Purpose

    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mNeonGreen
    

    Use

    Tags

    RIPGLIN

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDM071

    Plasmid

    #216817

    Purpose

    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mCardinal
    

    Use

    Tags

    RIPGLIN

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. human KANK2 Ankyrin Repeats pET151

    Plasmid

    #164054

    Purpose

    Expresses human KANK2 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-At-TRL

    Plasmid

    #32242

    Depositor

    Stan Fields, Jay Hesselberth

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    A. thaliana tRNA ligase (RNL Mustard Weed)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polmyerase

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge