33,434 results
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Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only
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SuperNova Green/ pRSETB
Plasmid#124839Purposebacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent proteinDepositorInsertSuperNova Green
TagsNo tagExpressionBacterialAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGNW2
Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD112
Plasmid#119893PurposepNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630DepositorTypeEmpty backboneExpressionBacterialAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLBH559_Tet-HisCas14a1Locus
Plasmid#112502PurposeExpresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14a1
UseCRISPRTags6xHis-TEVExpressionBacterialPromoterTetAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET32-MCM2/7
Plasmid#116949PurposeExpresses human MCM2 and human MCM7DepositorAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.011
Plasmid#108529PurposeTetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.DepositorInsertTetR + PTet*-YFP
ExpressionBacterialAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE3G-MYCN
Plasmid#104542PurposePiggyBac vector encoding doxycycline-inducible human MYCNDepositorInsertMYCN proto-oncogene, bHLH transcription factor (MYCN Human)
ExpressionBacterial and MammalianPromoterTRE3GAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
TagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV762-Peredox-mCherry
Plasmid#90217PurposeFluorescent reporter for NADH:NAD+ ratios in Mycobacteria.DepositorInsertCodon Optimized Peredox-mCherry
UseShuttle vectorExpressionBacterialPromotergroELAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRExpressionBacterialPromoterPLspAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNCS-mCyRFP1
Plasmid#84545PurposeBacterial expression of mCyRFP1DepositorInsertmCyRFP1
TagsHisExpressionBacterialAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAS1yl
Plasmid#73226PurposeConstitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cellsDepositorInsertsCas9
sgRNA
UseCRISPR; In y. lipolyticaExpressionBacterialPromoterunknownAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-MSP1D1delltaH4
Plasmid#71713PurposeMembrane scaffold protein (MSP), helix 4 deletionDepositorAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b His6-ecDHFR (WT)
Plasmid#73188Purposebacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)DepositorAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEERM3 Km
Plasmid#64025PurposePlasmid for integration into neutral site of Synechocystis 6803DepositorPublicationTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
Plasmid#61590PurposeExpresses human TRABID (OTU+AnkUBD domains) in E. coli.DepositorInsertTRABID (ZRANB1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-244 and aa 698-708.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKTrfp
Plasmid#59772PurposepKT230 derivative; PBAD_rfp; cmRDepositorInsertrfp
ExpressionBacterialPromoterpBADAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by