29,691 results

1. pTU1-A-SP44-sfGFP

   Plasmid

   #163757

   Purpose

   Expresses Streptomyces codon-optimised sfGFP

   Depositor

   Simon Moore, Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   SP44

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS4333 yeTadA1.0-T7 RNAP

   Plasmid

   #137735

   Purpose

   pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

   Depositor

   Christina Smolke

   Article

   Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

   Insert

   yeTadA1.0-T7 RNAP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   E366G (See depositor comment below)

   Promoter

   Available since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-Tight-Puro_Sox10

   Plasmid

   #46536

   Purpose

   A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Sox10 (Sox10 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ki67∆FHA-mNeonGreen-IRESpuro2

   Plasmid

   #183739

   Purpose

   Expresses Ki67∆FHA-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67_lacking FHA (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking FHA)

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOlyA(E69A)

   Plasmid

   #132392

   Purpose

   Mutant version of OlyA that senses sphingomyelin without or with cholesterol

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A point mutant (E69A)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, E69A, C94S, S151C

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

   Plasmid

   #183741

   Purpose

   Expresses Ki67 (8 repeats+LR)-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67 LR domain + 8 Ki-67 repeats

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK092

   Plasmid

   #183146

   Purpose

   pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB1 (CK2alpha)

   Plasmid

   #27083

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. NEDD4

   Plasmid

   #25141

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   nedd4.0001.0152 (NEDD4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pD454-SR rat Alpha-synuclein

    Plasmid

    #89074

    Purpose

    bacterial expression of rat Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pMRE154

    Plasmid

    #118504

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST44

    Plasmid

    #64007

    Purpose

    polycistronic cloning vector for pST44 plasmid suite

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRetroX-PTuner DD-linker-M.EcoGII

    Plasmid

    #122082

    Purpose

    Express M.EcoGII with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of M.EcoGII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-UAS1B8-Leum

    Plasmid

    #44316

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-Leum promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-Leum

    Available since

    April 18, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pZac2.1 gfaABC1D-eGFP

    Plasmid

    #176861

    Purpose

    Expresses cytosolic eGFP in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMYC-GFP

    Plasmid

    #42142

    Depositor

    Olufunmilayo Olopade

    Article

    Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

    Insert

    c-Myc (MYC Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFV2-HA

    Plasmid

    #187082

    Purpose

    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

    Depositor

    Steven Vik

    Article

    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

    Insert

    atpBEFHAGDC
    

    Use

    Tags

    6-His beta subunit (atpD) and HA tag subunit a (a…

    Expression

    Bacterial

    Mutation

    All Cys codons changed to Ala.

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7RNAP-Q737P

    Plasmid

    #175356

    Purpose

    Support plasmid for T7_ori plasmids

    Depositor

    Sven Panke

    Article

    Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

    Insert

    T7RNAP-Q737P
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q737P

    Promoter

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28CLY1

    Plasmid

    #21760

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible one SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMpGWB402-er-mCherry

    Plasmid

    #186288

    Purpose

    Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    er-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge