32,120 results

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pUC19-Top2A-5X Gly-Venus-T2A-Hygro

Plasmid

#211115

Purpose

For tagging the C-term of Top2A with Venus

Depositor

Job Dekker

Article

Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

Inserts

5' C-term Top2A homology arm (TOP2A Human)
Venus
T2A
Blastocidin Resistance
5' C-term Top2A homology arm (TOP2A Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

n/a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTCF120 H-2D(b)

Plasmid

#180454

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

H-2D(b)

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP2

Plasmid

#188897

Purpose

Express 6xHis-Tag miniGFP2 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCutamp

Plasmid

#140632

Purpose

Plasmid-curing in Escherichia coli by targeting the AmpR promoter

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe-SH1446

Plasmid

#112507

Purpose

Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

Depositor

Simon Newstead

Article

Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

Insert

POT family transporter

Tags

C terminal TEV GFP octa-His affinity tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b His6-FKBPF36V

Plasmid

#73180

Purpose

Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

Depositor

Thomas Wandless

Article

Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

Insert

FKBP(F36V) (FKBP1A Human)

Tags

His 6x

Expression

Bacterial

Mutation

F36V

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
pET41a iGluh

Plasmid

#119830

Purpose

Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluh

Tags

GST

Expression

Bacterial

Mutation

E25A

Promoter

T7

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-Cdc34a

Plasmid

#18674

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Cdc34A (CDC34 Human)

Tags

His

Expression

Bacterial

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pAWP78-PVCpnf_pvc13-Ad5RGDPK7

Plasmid

#198291

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDD112

Plasmid

#119893

Purpose

pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCA1.332 pDUOTK1-L1

Plasmid

#162351

Purpose

Level 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiency

Depositor

Alistair McCormick

Article

Gale et al Front Microbiol. 2021 Jan 15;11:624011.

Inserts

eYFP
LacZ alpha
mTagBFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHA2

Plasmid

#85710

Purpose

This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Ulrich Koszinowski, Martin Messerle

Article

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Expression

Bacterial

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAB_14-3-3

Plasmid

#109904

Purpose

Protein expression and purification of YWHAB_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAB_14-3-3 (YWHAB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCarmine in pRSETB

Plasmid

#109484

Purpose

bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Tags

N-terminal histidine tag in vector

Expression

Bacterial

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINB-HOIL-1 full-length (1–510)

Plasmid

#193858

Purpose

Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

Depositor

Bernhard Lechtenberg

Article

Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

Insert

HOIL-1 (RBCK1 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

Codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-153

Plasmid

#118567

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmCherry1

Plasmid

#89986

Purpose

Long stokes-shift variant of red fluorescent protein mCherry

Depositor

Robert Campbell

Article

Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

Insert

LSSmCherry1

Tags

6xHis-T7-Xpress

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEDA5_GFPmut3_Y66H

Plasmid

#80085

Purpose

Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

Depositor

Timothy Whitehead

Article

Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

Insert

GFPmut3_Y66H

Tags

6x-His tag

Expression

Bacterial

Mutation

changed Tyrosine 66 to Histidine

Promoter

proB

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDuet TDP43 WT

Plasmid

#27462

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Tags

GST

Expression

Bacterial

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pBFC0687

Plasmid

#186692

Purpose

ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsB
tnsC
tniQ
Cas12k
Sh_2xBsaI_NT (Guide Stuffer) gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDDRFP-A1

Plasmid

#36289

Depositor

Robert Campbell

Article

Alford et al Chem Biol. 2012 Mar 23;19(3):353-60.

Insert

Dimerization dependent RFP A1

Tags

His tag

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pRK1035

Plasmid

#8832

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease

Tags

His and MBP (cleaved)

Expression

Bacterial

Mutation

synthetic gene with codons optimized for E. coli

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Expression

Bacterial

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

Plasmid

#182390

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
OmpA-mCherry

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_NoMTS_L6_pET28a+

Plasmid

#60012

Purpose

Expresses TFAM No MTS L6 mutant in bacterial cells

Depositor

David Chan

Article

Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

Insert

Human TFAM No MTS (TFAM Human)

Tags

6xHis

Expression

Bacterial

Mutation

Missing 1st 42 aas (Mitochondrial targeting seque…

Promoter

T7

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET28_WT-LGP2 (codon optimized)

Plasmid

#248163

Purpose

Expresses full-length human LGP2 in E. coli

Depositor

Smita Patel

Article

Lee et al Nucleic Acids Res. 2024 Jan 11;52(1):355

Insert

DExH-box helicase 58 (DHX58) (DHX58 Human)

Tags

6xHis, SUMO

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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