Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

33,902 results

Showing: 1921 - 1940 of 33902 results

  1. ABL1_HUMAN_D0

    Plasmid
    #79727
    Purpose
    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    ABL1 (ABL1 Human)
    Tags
    His10-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  5. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Publication
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Tags
    6xHis tag, (-30)dGFP, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

    Enlarge

  12. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Publication
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    53731-plasmid-map-sequence-id-83909

    Enlarge

  14. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Publication
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 5-108, and contain…
    Promoter
    IRES and tac
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  16. pJS167

    Plasmid
    #48881
    Purpose
    Positive feedback plasmid from oscillator
    Depositor
    Jeff Hasty
    Publication
    Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi
    Inserts
    araC gene with ssrA tag
    monomeric yeast-enhanced green fluorescent protein
    Use
    Synthetic Biology
    Tags
    ssrA (TSAANDENYALAA)
    Expression
    Bacterial
    Promoter
    Para/lac
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    48881-plasmid-map-sequence-id-71862

    Enlarge

Showing: 1921 - 1940 of 33902 results