30,738 results

1. pTHSSe_45

   Plasmid

   #109243

   Purpose

   pSC101 var3 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE145

   Plasmid

   #118497

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT-WT-HA-GFP11

   Plasmid

   #177719

   Purpose

   ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET20b_Pa-PirA

   Plasmid

   #128956

   Purpose

   expresses Pseudomonas aeruginosa PirA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)
   

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-CL7-2NLS-SpyCas9(dS)-A22p3star

   Plasmid

   #222970

   Purpose

   Bacterial expression of A22p-fused SpyCas9 construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His-CL7-2NLS-SpyCas9(dS)-A22p3
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-HA-BI-AR

   Plasmid

   #171234

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor (AR Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX 6P1 CDK2 (552)

   Plasmid

   #61845

   Purpose

   bacterial expression of GST CDK2 WT

   Depositor

   Jonathon Pines

   Insert

   CDK2 (CDK2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pExp-DsbC

   Plasmid

   #112564

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-DsbC and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTHSSe_47

   Plasmid

   #109245

   Purpose

   pSC101 var5 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-tetoff-mVenus-PICdc42

    Plasmid

    #91876

    Purpose

    PI(WT): Photo inhibitable (blue light sensitive)

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Vav2 (VAV2 Human)
    

    Tags

    mVenus

    Expression

    Bacterial and Mammalian

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET24a-ncOGT-FL

    Plasmid

    #190821

    Purpose

    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E.coli

    Promoter

    T7

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pT3TS-nCas9n

    Plasmid

    #46757

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    T3

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLC-217

    Plasmid

    #73163

    Purpose

    Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-relE (relE E. coli (bacteria))
    

    Expression

    Bacterial

    Promoter

    PrhaB

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYHY1

    Plasmid

    #215926

    Purpose

    E. coli-Bacteroides shuttle vector.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Expression

    Bacterial

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE-Tn7-167

    Plasmid

    #118579

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-3c-rhVTN-N

    Plasmid

    #30225

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin with N terminus cleavage (VTN Human)
    

    Expression

    Bacterial

    Mutation

    deleted amino acids: 20*-62

    Available Since

    May 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYTK-DN1

    Plasmid

    #180282

    Purpose

    Cas9 cassette (TU1)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS - pPGK1 - Cas9 - tPGK1 - ConR1
    

    Expression

    Bacterial

    Promoter

    pPGK1

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIOX

    Plasmid

    #51300

    Purpose

    E.coli expression vector for His8-SUMO tagged recombinant protein

    Depositor

    Andrea Mattevi

    Type

    Empty backbone

    Tags

    His8 + SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pet-MBP

    Plasmid

    #209184

    Purpose

    Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Type

    Empty backbone

    Tags

    HisX6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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