4,932 results
-
Plasmid#224344PurposeExpresses human KDAC8 (HDAC8) H143A in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionInsectMutationHistidine 143 mutated to alaninePromoterPolyhedrinAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC8 D101E
Plasmid#224350PurposeExpresses human KDAC8 (HDAC8) D101E in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
TagsTEV-cleavable His6ExpressionInsectMutationAspartate 101 mutated to glutamatePromoterPolyhedrinAvailable SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUASTB
Plasmid#18944DepositorInsertphiC31 attB
ExpressionInsectAvailable SinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKSB-609 U6-sgRNA1 ATCC GGAA
Plasmid#173671PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE3c
Plasmid#17463Purposemodified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB
Plasmid#52890Purpose"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.DepositorInsertsExpressionInsectAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EPOP
Plasmid#125170PurposeExpresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-TAK1-V5-His
Plasmid#63753PurposeExpresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRmHA3-CH2F2-CG9866
Plasmid#52286PurposeExpresses Drosophila Ataxin-7DepositorAvailable SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC153-20XUAS-IVS-B2::PEST
Plasmid#32134DepositorInsertB2::PEST
ExpressionInsectAvailable SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVL1393-MAL-SYK
Plasmid#157990PurposeExpresses SYK in insect cell (sf9)DepositorInsertSpleen tyrosine kinase (SYK Human)
TagsMet-Ala-Leu and add Leu,Glu residues +His tagExpressionInsectAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAFW-Ago1
Plasmid#50553PurposeExpression of FLAG tagged Ago1 in S2 cellDepositorAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-Ala11-GFP
Plasmid#126654PurposeInducible expression of Draper-Ala11-GFP mutant in S2 cellsDepositorInsertDraper cytoplasmi Tyr residues mutated to Ala
ExpressionInsectMutationCytoplasmic Tyr residues to AlaAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT Shark-TagBFP
Plasmid#126658PurposeInducible expression of Shark-TagBFP in S2 cellsDepositorInsertShark
ExpressionInsectAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
TagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCaST-elav-VP16AD
Plasmid#15308DepositorInsertVP16AD-ZIP+
ExpressionInsectAvailable SinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCoHygro L16_shRNA of spliced Copia
Plasmid#71389Purposeknock down expression of spliced Copia in Drosophila melanogasterDepositorInsertCopia
ExpressionInsectPromoterU6Available SinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Luc
Plasmid#21604DepositorInsertUAS-hsp70 luciferase
ExpressionInsectAvailable SinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-p65-3xP3-RFP
Plasmid#127565PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.DepositorInsertp65
UseCRISPRExpressionInsectAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPHK1
Plasmid#73260PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-mtdT-3XHA
Plasmid#24355DepositorInsertmtDT-3XHA
Tags3XHA and mtdTExpressionInsectAvailable SinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBFv-UAS3-GFP1-10
Plasmid#138398PurposeExpresses split GFP fragment (GFP1-10)DepositorInsertGFP1-10
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Consensus
Plasmid#176664PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterChicken B-ActinAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEX-Antenna-GDI Rac1
Plasmid#83359PurposeExpression of Antenna-Rac1DepositorInsertRac1 (RAC1 Human)
TagsFRET Antenna (mCerulean-cpVenus) and HisExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2
Plasmid#165831PurposeEBFP2blue fluorescent protein CDSDepositorInsertEBFP2
ExpressionInsectAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
act-AsCpf1
Plasmid#73917Purposeexpression plasmid of AsCpf1DepositorInserthAsCpf1
TagsNLS-3xHAExpressionInsectPromoteract5CAvailable SinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP6
Plasmid#178072PurposeInsect cell expression of GCP6DepositorInsertGCP6 (TUBGCP6 Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-TRAF6-V5-His
Plasmid#63752PurposeExpresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL
Plasmid#83342Purposefor Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-DsRedSV40 GGGG AAGA
Plasmid#174532Purpose3xP3-DsRed fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-DsRed BsaI cassette
ExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-mTurqSV40 GGGG AAGA
Plasmid#174533Purpose3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-mTurquoise BsaI cassette
ExpressionInsectPromoter3xP3Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-RhoA Q63L
Plasmid#81058Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectPromoterDmel Hsp70BbAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-NbVHH05-GFP
Plasmid#171576PurposeExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembraneDepositorInsertCD8-NbVHH05-GFP (UBE2J1 Human)
TagsCD8 and GFPExpressionInsectPromoterfly actin5C promoterAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only