4,908 results

1. pCRISPaint-3xP3-GFP

   Plasmid

   #130277

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSTARR-seq_fly

   Plasmid

   #71499

   Purpose

   flySTARR-seq screening vector with DSCP core promoter.

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Flystarr-seq screening vector

   Tags

   sgGFP (SuperGlo, Qbiogene, Inc)

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-3xP3-RFP

   Plasmid

   #130276

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   3xP3-RFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBPGAL4.2::p65Uw

   Plasmid

   #26229

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   GAL4::p65 (RELA Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL His-p97

   Plasmid

   #113510

   Purpose

   Expression of His-p97 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97 (VCP Human)
   

   Use

   Tags

   6*His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mCherry::LANS4

   Plasmid

   #60785

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS4
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

   Plasmid

   #73164

   Purpose

   multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

   Depositor

   Bruce Edgar

   Article

   Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

   Insert

   mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
   

   Use

   Tags

   mRFP1, GFP

   Expression

   Insect

   Mutation

   Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAW-Nb127D01-mCherry

   Plasmid

   #171573

   Purpose

   Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-mCherry (CXCR2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   fly actin5C promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHD-SPARC2-D-LexA::p65

   Plasmid

   #133562

   Purpose

   Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pE1n

    Plasmid

    #17441

    Purpose

    modified pENTR vector for N-terminal STREP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    STREP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOEM1_pCMV:hShh-pPH:vsvged

    Plasmid

    #111156

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Nacu et al Nature. 2016 May 19;533(7603):407-10. do

    Insert

    hShh (SHH Human)
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4

    Plasmid

    #217895

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4)

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFL-EGFP-3xFlag-DEST

    Plasmid

    #134903

    Purpose

    Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Type

    Empty backbone

    Use

    Tags

    3x-Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINE-3C-eGFP

    Plasmid

    #41125

    Depositor

    Ray Owens

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFlpStop-HDR-UAS-2.1-tdTom

    Plasmid

    #89148

    Purpose

    FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

    Depositor

    Tom Clandinin

    Article

    Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    5XUAS, 3XP3-dsRed

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pECIA14

    Plasmid

    #47051

    Purpose

    This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

    Depositor

    Chris Garcia

    Article

    Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

    Type

    Empty backbone

    Use

    Secreted expression in drosophila culture

    Tags

    Alkaline Phosphatase (human, placental), Flag, HR…

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    Aug. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHACK-GAL4>QF2

    Plasmid

    #80275

    Purpose

    GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162387

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac1-pGC-A

    Plasmid

    #186626

    Purpose

    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

    Depositor

    Debra Hansen

    Article

    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

    Insert

    NPR1 (NPR1 Human)
    

    Use

    Tags

    hemagglutinin (HA) signal peptide + His10-tag + T…

    Expression

    Insect

    Mutation

    deleted amino acids 1-32

    Promoter

    polyhedring

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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