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Plasmid#149610PurposeGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.DepositorInsertGateway cassette (ccdb + Chlor.R.)
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4
Plasmid#217893PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)DepositorUseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-MCS
Plasmid#52908PurposeAedes aegypti polyubiquitin promoter followed by MCSDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAe aegptyi polyubiquitinAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
U6b
Plasmid#117222PurposeExpresses gRNA in Aedes aegyptiDepositorInsertHR U6b promoter-gRNA
UseSynthetic BiologyTagsExpressionInsectMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMycDam
Plasmid#59218PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli DamDepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectMutationPromoterDrosophila heat-shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Omega 1
Plasmid#118046PurposeGoldenBraid2.0 Omega 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)
Plasmid#48297DepositorTypeEmpty backboneUseTagsStrepII and msfGFPExpressionInsectMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRTagsExpressionBacterial and InsectMutationPromoter3XP3 eye-expression PromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-mCherry-Patronin
Plasmid#31753DepositorInsertPatronin (Patronin Fly)
UseTagsmCherryExpressionInsectMutationPromoterMetallothioneinAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3
Plasmid#59038Purposefor baculovirus expression of mouse CAMSAP3 in Sf9 cellsDepositorInsertCAMSAP3 (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6L
Plasmid#198409PurposeExpresses FLAG-tagged human IntS6LDepositorInsertIntegrator complex subunit 6 like (INTS6L Human)
UseTags3x FLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-TuSC
Plasmid#178079PurposeInsect cell expression of gamma-TuSCDepositorUseTagsTEV-His6ExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPLp10
Plasmid#170462PurposeEarlyBac insect cell expression systemDepositorInsertNew vector
UseTagsExpressionInsectMutationPromoterdHsp70Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI-KDAC8 H143A
Plasmid#224344PurposeExpresses human KDAC8 (HDAC8) H143A in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationHistidine 143 mutated to alaninePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacI-KDAC8 D101E
Plasmid#224350PurposeExpresses human KDAC8 (HDAC8) D101E in insect cellsDepositorInsertKDAC8 (HDAC8 Human)
UseTagsTEV-cleavable His6ExpressionInsectMutationAspartate 101 mutated to glutamatePromoterPolyhedrinAvailable sinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
UseTagsFLAG purification tag and eGFPExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-2xFlag-mCherry-Msp300KASH
Plasmid#170807PurposeExpresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesisDepositorInsert2xFLAG/mCherry-Msp300KASH
UseTags2xFLAG-mCherry and Msp300KASHExpressionInsectMutationPromoter5xUAS + hsp70 minimal promoterAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-3xFLAG-hSpCas9
Plasmid#162161PurposeExpresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTags3xFLAGExpressionInsectMutationPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7-pAc5.1 mCherry EcoRV
Plasmid#206490PurposeSchneider cell imagingDepositorTypeEmpty backboneUseTagsmCherryExpressionInsectMutationPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only