4,908 results

1. pBIG2abc

   Plasmid

   #80617

   Purpose

   pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1180-HR-PUbEBFP2-NLS

   Plasmid

   #176682

   Purpose

   Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   EBFP2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-mCh-Tub

   Plasmid

   #24288

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Alpha-Tubulin (alphaTub84B Fly)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pAcBac1-GFP(39TAG)

   Plasmid

   #217360

   Purpose

   Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   Enhanced green fluorescent protein
   

   Use

   Baculoviral

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Y39TAG

   Promoter

   CMV

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. NHR1+2-Flag pBacPAK8-3

   Plasmid

   #17324

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Neuralized-like (Neurl1a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pAct:dCas9-GCN4

   Plasmid

   #78899

   Purpose

   Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-10XGCN4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1 PIK3R1

   Plasmid

   #17743

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pUASp YFP Rab5 WT

   Plasmid

   #53468

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab05 (Rab5 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

9. 10XSTAT92E–luciferase

   Plasmid

   #37393

   Depositor

   Norbert Perrimon

   Article

   Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

   Insert

   SOCS36E enhancer fragment (Socs36E Fly)
   

   Use

   Luciferase; Jak/stat reporter construct

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Hsp70

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG2abcde

    Plasmid

    #80619

    Purpose

    pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOmnibac_ZZ_linker_ybbR_Tau MTBD

    Plasmid

    #159719

    Purpose

    Expresses microtubule-binding domain of tau (amino acids 242-367) in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    Tau microtubule-binding domain only (amino acids 242-367) (MAPT Human)
    

    Use

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Mutation

    Only amino acids 242-367

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

    Plasmid

    #217511

    Purpose

    KD Recombinase dependent conditional smGdP-10XHA cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
    

    Use

    Tags

    smGdP-10XHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

    Plasmid

    #217510

    Purpose

    KD Recombinase dependent conditional smGdP-10XV5 cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
    

    Use

    Tags

    smGdP-10XV5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSAR-Cre-F2A-I-SceI

    Plasmid

    #62302

    Purpose

    Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    Cre recombinase, I-SceI nuclease
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pE6c

    Plasmid

    #17466

    Purpose

    modified pENTR vector for C-terminal EYFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    EYFP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. gRNAy

    Plasmid

    #168294

    Purpose

    Expresses gRNA targeting the yellow gene in Drosophila

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    U6.3-gRNA[y]-scaffold
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162384

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINN-GFP

    Plasmid

    #53541

    Purpose

    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system

    Depositor

    Ray Owens

    Insert

    GFP
    

    Use

    Tags

    N-His-GFP

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG2abcd

    Plasmid

    #80618

    Purpose

    pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pOPINVL

    Plasmid

    #26040

    Depositor

    Ray Owens

    Article

    Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

    Insert

    u-LacZ
    

    Use

    Tags

    u-phosphatase secretion leader

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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