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5,017 results

Showing: 561 - 580 of 5017 results

  1. pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

    Plasmid
    #185447
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF1 (TERF1 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185447-plasmid-map-sequence-id-364982

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  6. pDyn1

    Plasmid
    #64067
    Purpose
    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).
    Depositor
    Andrew Carter
    Article
    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:
    Insert
    DYNC1H1 (DYNC1H1 Human)
    Use
    Cre/Lox
    Tags
    His-ZZ-TCS-SNAPf
    Expression
    Insect
    Mutation
    Codon optimized for Sf9
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    64067-plasmid-map-sequence-id-107133

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  14. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  15. pExpreS2-1-C3-PA-10H-SIII

    Plasmid
    #175443
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    175443-plasmid-map-sequence-id-360135

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  16. pUASp-GBP-Tbro

    Plasmid
    #179995
    Purpose
    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.
    Depositor
    Graydon Gonsalvez
    Article
    Baker et al Development. 2021 Nov 15;148(22). pii: 2
    Insert
    GBP nanobody
    Tags
    Trbo
    Expression
    Insect
    Promoter
    UAS-Gal4
    Available Since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    179995-plasmid-map-sequence-id-357336

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  17. IR25a-T2A-QF-AD

    Plasmid
    #162526
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162526-plasmid-map-sequence-id-354666

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  20. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available Since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

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Showing: 561 - 580 of 5017 results