4,908 results

1. pw35Gal4

   Plasmid

   #25901

   Depositor

   Craig Montell

   Article

   Moon et al Curr Biol. 2009 Oct 13. 19(19):1623-7.

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pUASattB-PKA-SPARK

   Plasmid

   #106922

   Purpose

   Express PKA-SPARK, an activity reporter of protein kinase A in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   PKA-SPARK
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT-EGFP-Actin5c

   Plasmid

   #15312

   Depositor

   Ron Vale

   Article

   Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

   Insert

   Actin5c (Act5C Fly)
   

   Use

   Tags

   EGFP and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pIEx4-Dio-GFP

   Plasmid

   #198278

   Purpose

   Cre reporter for insect cells; double-floxxed-EGFP

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pIEx4-Dio-GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   IE1 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pScarlessHD-C-3x127D01-DsRed

   Plasmid

   #171578

   Purpose

   For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3x127D01 (CXCR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pE5c

   Plasmid

   #17465

   Purpose

   modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   ECFP

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pActPL-Gal4DBD

   Plasmid

   #15304

   Depositor

   Benjamin White

   Article

   Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

   Insert

   ZIP- Gal4DBD (GAL4 )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOCC7

   Plasmid

   #118876

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ptubP-QS

   Plasmid

   #24352

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QS
   

   Use

   Tags

   white gene

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AE-Polyubiquitin-GCaMP6s

    Plasmid

    #106868

    Purpose

    Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

    Depositor

    Omar Akbari

    Article

    Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

    Insert

    AE-Polyubiquitin-GCAaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti polyubiquitin (PUb)

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL3-Gateway-DSCP

    Plasmid

    #71506

    Purpose

    flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid

    #169182

    Purpose

    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    His6-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

    Plasmid

    #55216

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. T7-pAc5.1 mCherry EcoRV

    Plasmid

    #206490

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDmVasa

    Plasmid

    #165783

    Purpose

    Drosohphila vasa germline promoter and 5'UTR

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Vasa promoter
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAFW-Ago2

    Plasmid

    #50554

    Purpose

    Expression of FLAG tagged Ago2 in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

    Insert

    Argonaute2 (AGO2 Fly)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid

    #214139

    Purpose

    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Use

    Tags

    2xSTII

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

    Plasmid

    #172066

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. RhoC FLARE.sc mCer, mVenus - CA

    Plasmid

    #65424

    Purpose

    RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus, Q63L in RhoC

    Promoter

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. RhoC FLARE.sc mCer, mVenus - DN

    Plasmid

    #65423

    Purpose

    RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus, T19N in RhoC

    Promoter

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only