5,046 results

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pAC8_GWs-Lox (VE5589)

Plasmid

#163769

Purpose

Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

no insert

Tags

no tag

Expression

Insect

Promoter

PH

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8_PH-GST-Nter-GWs-Lox (VE5586)

Plasmid

#163766

Purpose

Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

N-terminal GST tag

Tags

GST Tag

Expression

Insect

Promoter

PH

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.3

Plasmid

#190797

Purpose

aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1053B

Plasmid

#184007

Purpose

Expresses arrays of gRNA targeting hh promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNAhh1 - U6c:sgRNAhh2

Expression

Insect

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_6xHis-TEV-mCh-NDP52

Plasmid

#187829

Purpose

Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHisTag, TEV, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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RAT

Plasmid

#187416

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Tags

6xHis-TEV

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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ReactionC_pBIG1a_ATG7_ATG10

Plasmid

#190860

Purpose

Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG7 (ATG7 Human)
ATG10 (ATG10 )

Expression

Bacterial and Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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doraWT_rescue_construct

Plasmid

#190609

Purpose

Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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mTubulin NLS-mCherry-LEXY

Plasmid

#182597

Purpose

Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

mCherry

Tags

LEXY and NLS

Expression

Insect

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR IRFP-Bcd-LEXY

Plasmid

#182595

Purpose

Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

IRFP and LEXY

Expression

Insect

Promoter

maternal tubulin

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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p130Cas Y15F

Plasmid

#186154

Purpose

For expressing recombinant p130Cas in insect cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6 (Tev)

Expression

Insect

Mutation

15Y residues mutated to Alanine

Promoter

polyhedrin

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)

Plasmid

#185447

Purpose

Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TRF1 (TERF1 Human)
POT1 (POT1 Human)
TPP1 (TPP1 Human)
TIN2 (TINF2 Human)

Tags

MBP, YBBR, and ZZ

Expression

Insect

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOmniBac zz TEV YBBR TRF2

Plasmid

#185443

Purpose

Expresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Insert

TRF2 (TERF2 Human)

Tags

YBBR and ZZ

Expression

Insect

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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AeU6 3p3 GFP LgRNA

Plasmid

#182208

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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gRNAy

Plasmid

#168294

Purpose

Expresses gRNA targeting the yellow gene in Drosophila

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

U6.3-gRNA[y]-scaffold

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-MS2_V

Plasmid

#147737

Purpose

Insect Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-V5His6_D

Plasmid

#146093

Purpose

Insect Expression of DmPABPC1

Depositor

Elisa Izaurralde

Insert

DmPABPC1 (pAbp Fly)

Expression

Insect

Mutation

one non-silent mutation G26S compared to the sequ…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmFMR1_B

Plasmid

#145940

Purpose

Insect Expression of DmFMR1

Depositor

Elisa Izaurralde

Insert

DmFMR1 (Fmr1 Fly)

Expression

Insect

Mutation

Two amino acid mutations (N45D and G558R) and thr…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-TwinStrep-3C-DrHDAC6 (40-831)

Plasmid

#180226

Purpose

Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

Depositor

Patrick Matthias

Article

Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

Insert

Histone deacetylase 6 (hdac6 Zebrafish)

Tags

TwinStrep-3C

Expression

Insect

Promoter

polyhedrin

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

Plasmid

#178074

Purpose

Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT2A (MZT2A Human)

Tags

PreScission-mEGFP and ZZ-TEV

Expression

Insect

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-GCP5

Plasmid

#178071

Purpose

Insect cell expression of GCP5

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP5 (TUBGCP5 Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-NEDD1

Plasmid

#178073

Purpose

Insect cell expression of NEDD1

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

NEDD1 (NEDD1 Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-MZT1

Plasmid

#178075

Purpose

Insect cell expression of MZT1

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT1 (MZT1 Human)

Expression

Insect

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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RABGEF1A-c014

Plasmid

#175478

Purpose

Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.

Depositor

Opher Gileadi

Insert

RABGEF1 (RABGEF1 Human)

Tags

His6-Zbasic-TEV

Expression

Insect

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_ybbR_MAP7

Plasmid

#159718

Purpose

Expresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

MAP7 (Microtubule Associated Protein 7) (MAP7 Human)

Tags

ZZ affinity tag, ybbR tag

Expression

Insect

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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