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His-ZZ-TEV-SNAPf-BICDR1

Plasmid

#111858

Purpose

N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Use

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

R250Q, Optimised for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-lexAp65

Plasmid

#138393

Purpose

RMCE vector for generating C-terminal T2A-lexA-p65 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-lexAp65

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1067A1

Plasmid

#164847

Purpose

Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

Depositor

Omar Akbari

Article

Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

Insert

4 gRNAs and 3xP3-eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTattB-mtdT-3XHA

Plasmid

#24355

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

mtDT-3XHA

Use

Tags

3XHA and mtdT

Expression

Insect

Mutation

Promoter

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-SBP_J

Plasmid

#146621

Purpose

Insect Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-V5-SBP_J

Plasmid

#146633

Purpose

Insect Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pKSB- 3xP3-GFPsv40 GGGG AAGA

Plasmid

#174531

Purpose

3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes

Depositor

Eric Marois

Insert

3xP3-GFP BsaI cassette

Use

Tags

Expression

Insect

Mutation

Promoter

3xP3

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-PA-Rac1-I539E

Plasmid

#22026

Purpose

Expression of photoactivatable Rac1 ('lit' state mutant)

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))

Use

Tags

6X His

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV domain contains 'lit state' mutatio…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pE3c

Plasmid

#17463

Purpose

modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

6xMyc

Expression

Bacterial, Insect, Mammalia…

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pQUAST>stop>mCD8-GFP

Plasmid

#24361

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

mCD8-GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

Plasmid

#148087

Purpose

Insect Expression of DmPAN3-W707AG711E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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UAST-LbCas12a

Plasmid

#140621

Purpose

Gal4 dependent expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAST

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry::LANS1

Plasmid

#60784

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS1

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC153-20XUAS-IVS-B2::PEST

Plasmid

#32134

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pDmPolyUbiq

Plasmid

#165786

Purpose

Drosophila Polyubiquitin promoter, strongest promoter in the library

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

PolyUbiq

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHD-3xP3-dsRed-DattP

Plasmid

#133559

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Use

Tags

NLS-3xHA

Expression

Insect

Mutation

Promoter

act5C

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTriex-mVenus-PI-RhoA(Q63L)

Plasmid

#86978

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
RhoA (RHOA Human)

Use

Tags

6xHis and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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