4,931 results

1. CJ24 (let-7 minigene)

   Plasmid

   #17764

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   dme-let-7 minigene (let-7 Fly)
   

   Use

   Drosophila metallothionein gene promoter drives e…

   Expression

   Insect

   Available Since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pBiex PKC - Peptide 14 mCit mCer

   Plasmid

   #83565

   Purpose

   Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PKC alpha (PRKCA Human)
   

   Tags

   10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PI(DM)-Rac1(WT)dc1.FLARE

   Plasmid

   #87589

   Purpose

   PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   PBD
   Rac1
   

   Tags

   YPet and dTurq

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FoRC.PRm

   Plasmid

   #84587

   Purpose

   Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.

   Depositor

   Francesca Pignoni

   Article

   Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUASP Flag Rab32 WT

   Plasmid

   #44833

   Depositor

   Matthew Scott

   Insert

   Rab32 (Rab32 Fly)
   

   Use

   Drosophila

   Tags

   Flag

   Expression

   Insect

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. hs casper RhoL-N17

   Plasmid

   #17583

   Depositor

   Denise Montell

   Insert

   RhoL (RhoL Fly)
   

   Expression

   Insect

   Mutation

   N17

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pMT-GFP-Spindly

   Plasmid

   #24282

   Depositor

   Ron Vale

   Article

   Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

   Insert

   Spindly (Spindly Fly)
   

   Tags

   GFP

   Expression

   Insect

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pMCh-139

   Plasmid

   #92287

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

   Depositor

   Witold Filipowicz, Marina Chekulaeva

   Article

   Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

   Insert

   dGW182 NED (gw Fly)
   

   Tags

   NHA

   Expression

   Insect

   Mutation

   dGW182 NED: aa 205-490

   Promoter

   Ac5

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUAST YFP Rab2 WT

   Plasmid

   #58444

   Purpose

   expression of drosophila YFP-Rab2

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab2 (Rab2 Fly)
   

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pUASp YFP Rab6 WT

    Plasmid

    #37696

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab6 (Rab6 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    Nov. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pSTARR-seq_fly-NipB

    Plasmid

    #71502

    Purpose

    flySTARR-seq screening vector with NipB core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pattB-aubergine-ADH-gf

    Plasmid

    #69448

    Purpose

    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub

    Depositor

    Phillip Zamore

    Insert

    aubergine (aub Fly)
    

    Tags

    NA

    Expression

    Insect

    Mutation

    chr2L:10,998,626(−)T>G

    Promoter

    aubergine

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRmHa3-dKDM4A-HA2FL2

    Plasmid

    #20232

    Depositor

    Jerry Workman

    Article

    Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

    Insert

    dKDM4A (Kdm4A Fly)
    

    Tags

    FLAG2 and HA2

    Expression

    Insect

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

14. EFNA1A

    Plasmid

    #25358

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    efna1a.017.171 (EPHA1 Human)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. UAS-dally

    Plasmid

    #41175

    Depositor

    Norbert Perrimon

    Article

    Lin et al Nature. 1999 Jul 15;400(6741):281-4.

    Insert

    dally (dally Fly)
    

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pUAST YFP Rab6 DN

    Plasmid

    #58448

    Purpose

    expression of drosophila YFP-Rab6 dominant negative mutant

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab6 (Rab6 Fly)
    

    Tags

    YFP

    Expression

    Insect

    Mutation

    T26N (dominant negative)

    Promoter

    GAL4

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pUAST YFP Rab6 WT

    Plasmid

    #58447

    Purpose

    expression of drosophila YFP-Rab6

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab6 (Rab6 Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pFastBac-hDcr-K70A.

    Plasmid

    #89145

    Purpose

    Expression of helicase mutant of human Dicer in Sf9 cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    hDicer (DICER1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    K70A

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EKAR2G_design2_mTFP_227_Venus_173

    Plasmid

    #40016

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pFasBac+GFP-CAMSAP3 CC

    Plasmid

    #59042

    Purpose

    for baculovirus expression of mouse CAMSAP3 CC in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CC domain (Camsap3 Mouse)
    

    Tags

    His and eGFP

    Expression

    Insect

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BacPAK HF-Uch37C88A

    Plasmid

    #19426

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    Uch37 (UCHL5 Human)
    

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    C88A

    Available Since

    Jan. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

    Plasmid

    #79036

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Changed Lys 283 to Asp and Gln 287 to Asp

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HS-H3.3A d436-GFP

    Plasmid

    #8697

    Depositor

    Kami Ahmad

    Article

    Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

    Insert

    H3.3A truncated (His3.3A Fly)
    

    Tags

    GFP

    Expression

    Insect

    Mutation

    Deletion of amino acids 4-36.

    Available Since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAC-cwo

    Plasmid

    #33155

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

    Insert

    cwo (cwo Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pattB-synaptobrevin-4-QFBDMD-G4AD-hsp70

    Plasmid

    #46112

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QFBDMD-G4AD
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUASt- Crb intraGFP

    Plasmid

    #67525

    Purpose

    Expression of transmembrane and the intracellular domains of Crb with GFP.

    Depositor

    Marta Llimargas Casanova, Elisabeth Knust

    Insert

    crumbs (crb Fly)
    

    Use

    Drosophila

    Tags

    GFP

    Expression

    Insect

    Mutation

    aa97-2103 deleted (extracellular domain)

    Promoter

    hsp70

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pAct:MCP-VP64

    Plasmid

    #78904

    Purpose

    Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2:VP64
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    March 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EKAR2G_design1_mTFP_227_Venus_229

    Plasmid

    #39837

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

29. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[closed]

    Plasmid

    #71806

    Purpose

    Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[closed]
    

    Expression

    Insect

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUASTattb AaegOr4-C

    Plasmid

    #59794

    Purpose

    UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele C

    Depositor

    Leslie Vosshall

    Article

    McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

    Insert

    Aedes aegypti Or4 cDNA allele C
    

    Expression

    Insect

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUAST YFP Rab23 WT

    Plasmid

    #53484

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab23 (Rab23 Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pUASp YFP Rab18 DN

    Plasmid

    #53474

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab18 (Rab18 Fly)
    

    Tags

    YFP

    Expression

    Insect

    Mutation

    S19N

    Promoter

    GAL4

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pUASp YFP Rab18 CA

    Plasmid

    #37719

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    A64L (constitutive active)

    Promoter

    GAL4

    Available Since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pattB-synaptobrevin-2-G4BDMD-QFAD-hsp70

    Plasmid

    #46110

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    G4BDMD-QFAD
    

    Expression

    Insect

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSGE_662_S2-1-Tor_dCP

    Plasmid

    #71165

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    662_S2-1-Tor_dCP
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Promoter

    DSCP Drosophila synthetic core promoter

    Available Since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSGE_709_S2-1-Pal_dCP

    Plasmid

    #71162

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    709_S2-1-Pal_dCP
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Promoter

    DSCP Drosophila synthetic core promoter

    Available Since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBacPAK8-His-VHL-Flag(117-213)

    Plasmid

    #29501

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Von Hippel-Lindau (VHL Human)
    

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    VHL(117-213) only

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

38. Or85a-Gal4

    Plasmid

    #63052

    Purpose

    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or85a, using the promoter sequences of the gene cloned 5' of Gal4

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Sep 6;15(17):1548-53.

    Insert

    Drosophila melanogaster Or85a promoter
    

    Tags

    Drosophila ADH (aa's 1-18) fused to Yeast Ga…

    Expression

    Insect

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pattB-synaptobrevin-6-G4BDMD-QFADM2-hsp70

    Plasmid

    #46114

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    G4BDMD-QFADM2
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CJ40 (let-7 seed mutant perfect site)

    Plasmid

    #17773

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    let-7 seed mutant perfect site (let-7 Fly)
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    seed mutant

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only