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pSK25

Plasmid

#72853

Purpose

template for C-terminal double-FLAG tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

double Flag

Expression

Insect

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRIV-Gal4DBD:2xnMagHigh1

Plasmid

#182031

Purpose

Donor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1-p103'UTR

Use

Donor plasmid

Expression

Insect

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDW445

Plasmid

#8843

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Tags

biotin acceptor peptide

Expression

Insect

Available Since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

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ORCO-T2A-QF2

Plasmid

#162524

Purpose

Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

Depositor

Leslie Vosshall

Article

Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

Insert

Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm

Expression

Insect

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-HGP-v3-Nb127D01-hIgG

Plasmid

#171564

Purpose

Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)

Tags

BiP signal peptide and hIgG1-hinge-N297A-TEV site…

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-HGP-v3-NbVHH05-hIgG

Plasmid

#171565

Purpose

Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)

Tags

BiP signal peptide and hIgG1-hinge-N297A-TEV site…

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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CJ52 (cg1849 utr mut)

Plasmid

#17780

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg1849 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pUWR-CadTS

Plasmid

#58325

Purpose

Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

E-cadherin tension sensor

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only
pUASp-Egl-Trbo

Plasmid

#179993

Purpose

Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

Egl-Trbo

Tags

TurboID

Expression

Insect

Promoter

UAS-Gal4

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBDPR

Plasmid

#61598

Purpose

Transformation vector pBDPR

Depositor

John Carlson

Article

Koh et al Neuron. 2014 Aug 20;83(4):850-65. doi: 1

Type

Empty backbone

Use

Drosophila transformation vector

Expression

Insect

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 WWE-PARP

Plasmid

#192550

Purpose

E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKM610

Plasmid

#134218

Purpose

Baculovirus expression of CENP-R

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-R untagged

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]

Plasmid

#71810

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBacPak-HA-E(y)2

Plasmid

#52288

Purpose

Expresses Drosophila E(y)2

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

E(y)2 (e(y)2 Fly)

Tags

HA

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBiex1-SYNAC

Plasmid

#82710

Purpose

Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

Insert

SYNAC (ADCY2, ADCY5 Human)

Tags

FLAG, mCerulean, and mCitrine

Expression

Bacterial and Insect

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSK15

Plasmid

#72855

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac-Giardia Dicer

Plasmid

#89146

Purpose

Expression of Giardia Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

Giardia Dicer

Tags

His6-teve

Expression

Insect

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pVMG0146_Cq-U6-1_2xBbsI-gRNA

Plasmid

#169238

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

Cq-U6-1_2xBbsI-gRNA

Use

CRISPR

Expression

Insect

Promoter

CPIJ039653

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0149_Cq-U6-6_2xBbsI-gRNA

Plasmid

#169323

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0149_Cq-U6-6_2xBbsI-gRNA

Use

CRISPR

Expression

Insect

Promoter

CPIJ039596

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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44 pLIB_12HIS

Plasmid

#160186

Purpose

N-term 3C cleavable 12xHis tag for insect cell expression

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

12xHis

Expression

Insect

Promoter

polyhydrin

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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