78,292 results

1. pcDNA3.1-INSR-V5/HIS

   Plasmid

   #201980

   Purpose

   expression of human INSR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   INSR receptor tyrosine kinase (INSR Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hNTo2-qgRNA-pYJA5

   Plasmid

   #217782

   Purpose

   Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-Clover-HIF-2alpha Wildtype

   Plasmid

   #163371

   Purpose

   Express HA- and Clover-tagged HIF-2alpha wildtype plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
   

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV Promoter

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-CBE6c

   Plasmid

   #215821

   Purpose

   Express CBE6c (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-SpCas9-2xUGI (cas9 )
   

   Expression

   Mammalian

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-myc-HLA-B7

   Plasmid

   #135509

   Purpose

   Mammalian expression of myc-tagged HLA-B*07:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*07:02 (HLA-B Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM705 Gsq

   Plasmid

   #109356

   Purpose

   GsX chimera with Gαq C-terminus

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsq chimera
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1-FHC

   Plasmid

   #64874

   Purpose

   lentiviral vector with Flag, HA tags

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-LAMP1

   Plasmid

   #207787

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX303-ABI1-dCas9

   Plasmid

   #154474

   Purpose

   Expresses ABI1 fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ABI1 (ABI1 Mustard Weed)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Includes ABI1 aa 126-423, D143A

   Promoter

   CMV

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK-CMV-hNHERF2

    Plasmid

    #47801

    Purpose

    Expresses human NHERF2 in mammalian cells

    Depositor

    Randy Hall

    Article

    Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

    Insert

    NHERF2 (NHERF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSyn.iGluSnFR3.v857.SGZ

    Plasmid

    #178330

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ER-mNeonGreen

    Plasmid

    #137804

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    KDEL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG SMG1 WT

    Plasmid

    #199573

    Purpose

    Expresses FLAG-tagged WT SMG1

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    SMG1 (SMG1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human Integrin beta 2

    Plasmid

    #8640

    Depositor

    Timothy Springer

    Article

    Kishimoto et al Cell 1987 Feb 27;48(4):681-90.

    Insert

    Integrin beta 2 (ITGB2 Human)
    

    Expression

    Mammalian

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

15. CDH5-AbR-GFP

    Plasmid

    #122970

    Purpose

    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

    Depositor

    Budd A. Tucker

    Inserts

    CDH5p
    CopGFP
    Zeocin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EB1-tdTomato

    Plasmid

    #50825

    Purpose

    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Insert

    EB1 (Mapre1 Mouse)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEFL EGFP-TEADi

    Plasmid

    #140144

    Purpose

    TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    EGFP TEADi
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-pHluorin_M153R-CD63

    Plasmid

    #172117

    Purpose

    Fluorescent reporter for CD63-positive exosome secretion

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    pHluorin_M153R in a small extracelluar loop of CD…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CSF1R-V5/HIS

    Plasmid

    #201984

    Purpose

    expression of human CSF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    CSF1R receptor tyrosine kinase (CSF1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAX APP Swe/Ind

    Plasmid

    #30145

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    human APP 695 Swedish/Indiana mutation (APP Human)
    

    Expression

    Mammalian

    Mutation

    Swedish and Indiana; K595N, M596L and V642F

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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