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pDx_mScarlet-I3

Plasmid

#189757

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE8e-NG

Plasmid

#181754

Purpose

MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_KRAB

Plasmid

#126030

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with KRAB
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-c-Myc

Plasmid

#102626

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC proto-oncogene, bHLH transcription factor (MYC Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-CjCas9

Plasmid

#89752

Purpose

Expression of CjCas9 in mammalian cells

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cas9

Use

CRISPR

Tags

SV40-HA

Expression

Mammalian

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSynSRE-T-Luc

Plasmid

#60444

Purpose

Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

Depositor

Timothy Osborne

Article

Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

Insert

HMG-CoA synthase promoter (-324/-225)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-TOMM20-N-10

Plasmid

#55328

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

TOMM20 (TOMM20 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-Caveolin-C-10

Plasmid

#55008

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-TOMM20-N-10

Plasmid

#54282

Purpose

Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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