75,759 results

1. pIRES2-EGFP Mer

   Plasmid

   #14998

   Depositor

   Raymond Birge

   Article

   Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

   Insert

   Mer (Mertk Mouse)
   

   Use

   Tags

   IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. LbCas12a-crRNA

   Plasmid

   #213753

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a-crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-Tox-IRES-eGFP

   Plasmid

   #165427

   Purpose

   Expresses TOX in mammalian cells

   Depositor

   Anjana Rao, Patrick Hogan

   Article

   Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

   Insert

   TOX (Tox Mouse)
   

   Use

   Tags

   3xflag 5' of Tox

   Expression

   Mammalian

   Mutation

   Promoter

   5' LTR

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTREtight2

   Plasmid

   #19407

   Depositor

   Markus Ralser

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-puro TPR-Met

   Plasmid

   #10902

   Depositor

   Bob Weinberg

   Article

   Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

   Insert

   TPR-Met (MET, TPR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Constitutively active form of the c-Met receptor

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-mTagBFP2-T2A-iCre (v2)

   Plasmid

   #125822

   Purpose

   Monomeric TagBFP2 and iCre recombinase (version 2)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDG462

   Plasmid

   #100903

   Purpose

   SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ1651-sgTelomere(F+E)

   Plasmid

   #51024

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   Optimized sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

   Plasmid

   #107270

   Purpose

   Expresses Cas9 upon induction with doxicycline.

   Depositor

   Pablo Menéndez

   Article

   Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

   Inserts

   hSpCas9
   rtTA
   bGH polyA-SV40polyA
   

   Use

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-BLU-VIPR

    Plasmid

    #220497

    Purpose

    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-ABCB10-ChR2-eYFP

    Plasmid

    #206037

    Purpose

    Express ChR2 in mitochondria

    Depositor

    Lufang Zhou

    Article

    Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

    Insert

    ChR2
    

    Use

    Tags

    ABCB10 mitochondrial leading sequence and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-N-Myc_WT USP7

    Plasmid

    #131242

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 FLAG FBXW7alpha

    Plasmid

    #236470

    Purpose

    transient overexpression of FBXW7alpha in mammalian cells

    Depositor

    Michele Pagano

    Article

    Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

    Insert

    FBXW7alpha (FBXW7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid

    #200679

    Purpose

    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CMV_PsCatCh2.0-YFP

    Plasmid

    #186978

    Purpose

    Expresses PsCatCh2.0 channelrhodopsin in mammalian cells

    Depositor

    Adam Cohen

    Article

    Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

    Insert

    PsCatCh2.0-YFP
    

    Use

    Lentiviral

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVneo-DEST

    Plasmid

    #119746

    Purpose

    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pINDUCER dCas9-TET1CD

    Plasmid

    #101921

    Purpose

    The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DIO-Ef1a-TVA-2A-N2cG

    Plasmid

    #172360

    Purpose

    Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid

    #221359

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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