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pUBC_hsPUM1-mCherry

Plasmid

#107607

Purpose

Transient expression of Pumilio1, fused to mCherry in mammalian cells, under a hUBC promoter

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

hsPUM1-mCherry

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

human UBC

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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PaGFP-UtrCH

Plasmid

#26738

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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myc-Pex19

Plasmid

#127625

Purpose

N-terminal myc-tagged mouse Pex19

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Pex19 (Pex19 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-FADD

Plasmid

#78802

Purpose

To overexpress FADD in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

Insert

FADD (FADD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLAS3W-S100P

Plasmid

#125009

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

S100P (S100P Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GCaMP6f.WPRE.SV40

Plasmid

#100836

Purpose

AAV expression of ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV2, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Active Wnt11-V5

Plasmid

#43824

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT11 (WNT11 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV4 p65

Plasmid

#21966

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NFkB p65 subunit (RELA Human)

Expression

Mammalian

Mutation

This construct was the product of 3 subcloning pr…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Lck-mScarlet-I

Plasmid

#98821

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-2 myc

Plasmid

#11373

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

Plasmid

#130816

Purpose

Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

bJun-NFAST-NLS-DEVDG-mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1

Plasmid

#211578

Purpose

CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ALFA-bArr1

Plasmid

#201495

Purpose

Expression of the beta-arrestin1 containing a N-terminal ALFA tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

ALFA-bArr1 (ARRB1 Human)

Tags

ALFA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP delta

Plasmid

#12559

Depositor

Peter Johnson

Article

McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

Insert

CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)

Expression

Mammalian

Mutation

S2G introduced during cloning

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mAmetrine 1.1 FP

Plasmid

#52113

Purpose

a bicistronic IRES-mAtetrine containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-mAmetrine 1.1

Expression

Mammalian

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-mGFP-FTH1

Plasmid

#192800

Purpose

Stable expression of mGFP-FTH1 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

FTH1 (FTH1 Human)

Tags

monomeric EGFP (A206K)

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FilaminA-N-9

Plasmid

#55047

Purpose

Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

FilaminA (FLNA Human)

Tags

mCherry

Expression

Mammalian

Mutation

D2626H

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170995

Purpose

HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 WT

Plasmid

#195213

Purpose

Mammalian expression vector for GFP tagged NHERF1.

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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