78,024 results

1. pCMV-HA-PD-L1

   Plasmid

   #121492

   Purpose

   Expression of human PD-L1 WT in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1 (CD274 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Go1-CASE

   Plasmid

   #168123

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at L91/G92 within GNAO1

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176268

   Purpose

   Expression of dCas12f-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCas12f
   

   Use

   Lentiviral

   Tags

   HA-VPR-mCherry

   Expression

   Mammalian

   Mutation

   D326A, D510A

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

   Plasmid

   #205555

   Purpose

   Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-MEK5DD-HA

   Plasmid

   #65247

   Purpose

   Expression of HA tagged dominant active MEK5 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

   Insert

   MEK5DD (MAP2K5 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Ser311 to Asp, Ser315 to Asp

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPRDEnv

   Plasmid

   #1732

   Purpose

   This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

   Depositor

   Garry Nolan

   Insert

   Gag-Pol and Rev
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_SLC25A5

   Plasmid

   #132007

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A5 (SLC25A5 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLX_TRC311

   Plasmid

   #113668

   Purpose

   Gateway destination vector for lentiviral expression

   Depositor

   John Doench

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-hGal3

    Plasmid

    #73080

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin-3 (LGALS3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX208-cytoFLARE1.0-TF

    Plasmid

    #234513

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJT301 BxBi Lenti pEF-attB LSR GFP

    Plasmid

    #184944

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Michael Bassik, Patrick Hsu, Ami Bhatt

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    BxBi Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRESneo-PA-GFP-alpha Tubulin

    Plasmid

    #12297

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Tags

    PA-GFP

    Expression

    Mammalian

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105540

    Purpose

    AAV expression of EGFP-Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Neo_CAG-Cas9

    Plasmid

    #86698

    Purpose

    Cas9 expression vector for targeting to the AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    pCag

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-wtFAKSPARK2

    Plasmid

    #195263

    Purpose

    express wtFAKSPARK2

    Depositor

    Xiaokun Shu

    Article

    Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

    Insert

    FAKSPARK2 sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-PARP1-E988K

    Plasmid

    #211580

    Purpose

    CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains E988K mutation

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_AncBE4max_P2A_GFP

    Plasmid

    #112100

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_P2A_GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PER2:Luc Plasmid

    Plasmid

    #206875

    Purpose

    Per2 promoter luciferase reporter

    Depositor

    Michelle Farkas

    Article

    Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

    Insert

    Period 2 (Per2 Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIX403 RNaseH1

    Plasmid

    #237695

    Purpose

    Inducible lentiviral expression of RNaseH1

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    RNaseH1 (RNASEH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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