76,055 results

1. pC0079 RESCUE-S

   Plasmid

   #130662

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-IRES-Hygro_GCaMP6s

   Plasmid

   #164591

   Purpose

   Stable integration and expression of GCaMP6s in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Artichoke

   Plasmid

   #73320

   Purpose

   Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and EF1a

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAT-PB-CG2APtk

   Plasmid

   #86003

   Purpose

   homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   eGFP_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDZ2FE_Champ1_1-812

   Plasmid

   #105675

   Purpose

   Expresses Flag-tagged full-length human Champ1 protein

   Depositor

   Thanos Halazonetis

   Insert

   Champ1 (CHAMP1 Human)
   

   Use

   Tags

   2X Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1 SIRT1-FL

   Plasmid

   #105670

   Purpose

   Mammalian expression construct of mouse SIRT1 full length isoform

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDUAL SRBI (GFP)

   Plasmid

   #86979

   Purpose

   Lentiviral expression construct encoding SR-B1 and GFP from separate promoters

   Depositor

   Joe Grove

   Insert

   SCARB1 (SCARB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV/hPGK

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-alpha-tubulin

   Plasmid

   #49149

   Purpose

   expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

   Depositor

   Gia Voeltz

   Article

   Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

   Insert

   alpha tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. NSP6-FR-2Strep

    Plasmid

    #210317

    Purpose

    Expresses SARS-CoV-2 NSP6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM378

    Plasmid

    #173174

    Purpose

    Expresses C9-1 gRNA-12xMBS from hU6 promoter

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    C9-1 targeting gRNA-12xMBS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ORFtag_TetR-SD_Frame3

    Plasmid

    #220983

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CS2 Flag-Smad2 (3A)

    Plasmid

    #101763

    Purpose

    Expresses SMAD2 (3A) in mammalian cells

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Three Ser to Ala mutations in the phosphorylation…

    Promoter

    CMV

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. p23-NES-PguCas13b-msfGFP-NES-Flag

    Plasmid

    #165072

    Purpose

    overexpression of PguCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CamKIIa-jGCaMP8f-WPRE

    Plasmid

    #176750

    Purpose

    AAV-mediated expression of GCaMP8f under the CamKIIa promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIalpha

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. plex_307-ccdB-iRFP713

    Plasmid

    #201140

    Purpose

    pLEX_307 plasmid with iRFP713 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge