77,958 results

1. pAAV-Syn-ERT2-iCre-ERT2

   Plasmid

   #178059

   Purpose

   Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ERT2-iCre-ERT2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human synapsin 1 (Syn) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-mApple

   Plasmid

   #179383

   Purpose

   Lentiviral vector for expression of mApple

   Depositor

   Angus Johnston

   Article

   Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

   Insert

   mApple
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpCas9-NG

   Plasmid

   #138566

   Purpose

   Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. attB-GFP

   Plasmid

   #65521

   Purpose

   Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Expression

   Mammalian

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC0052-REPAIR non-targeting guide clone into pC0043

   Plasmid

   #103868

   Purpose

   Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   non-targeting guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LentiGuide-Neo

   Plasmid

   #139449

   Purpose

   Lentiviral vector with gRNA scaffold and neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   no sgRNA inserted; resistance gene: neoR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSico

   Plasmid

   #11578

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAG-mCherry-LacZshRNA

   Plasmid

   #73978

   Purpose

   LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the LacZ gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKM343

    Plasmid

    #69762

    Purpose

    Retroviral expression of CENP-I

    Depositor

    Iain Cheeseman

    Insert

    CENP-I (CENPI Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAcGFP1-N1-CAHS3

    Plasmid

    #90031

    Purpose

    To express GFP-tagged CAHS3 in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS3
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-HA-Nrxn1α-LNS2

    Plasmid

    #128106

    Purpose

    Express HA tagged Nrxn1α-LNS2

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS2 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAVU6+27-F30-2xdBroccoli

    Plasmid

    #66842

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-HA-Nrxn1α-LNS4

    Plasmid

    #128108

    Purpose

    Express HA tagged Nrxn1α-LNS4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS4 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-HA-Nrxn1α-LNS5

    Plasmid

    #128109

    Purpose

    Express HA tagged Nrxn1α-LNS5

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS5 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO1-puro-U6-sgRNA-TGM2

    Plasmid

    #69239

    Purpose

    U6 driven SpCas9 sgRNA expression for TGM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TGM2 sgRNA (TGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFH2.10_Gag-MCP-P2A-eGFP

    Plasmid

    #205525

    Purpose

    Express HIV Gag-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV AURKA-mTurq2

    Plasmid

    #157767

    Purpose

    Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge