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pCIS.MTRIA-NPY

Plasmid

#184613

Purpose

For expression of a fluorescent sensor for neuropeptide Y in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPY

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pZW6 (CK2alpha)

Plasmid

#27086

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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WT GBA pcDNA3.1

Plasmid

#188580

Purpose

Ectopic expression of wild-type GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sgAAVS1

Plasmid

#184403

Purpose

pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

AAVS1-specific sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCitrine

Plasmid

#34950

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA6-N-3XFLAG-Gsk3b

Plasmid

#123592

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

glycogen synthase kinase 3 beta (Gsk3b Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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HA-ISceID44A

Plasmid

#59424

Purpose

Expresses a catalytically inactive form of the endonuclease ISceI

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

endonuclease ISceI mutant (I-SceI )

Tags

HA tag

Expression

Mammalian

Mutation

changed aspartate 44 to alanine (D44A)

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Tags

FRB (T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-BRD2

Plasmid

#65376

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD2 (BRD2 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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mIFP12-Farnesyl-5

Plasmid

#56251

Purpose

Localization: Plasma Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Farnesyl (HRAS Human)

Tags

mIFP12

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-mScarlet-SARS-CoV-2-SL1 alone

Plasmid

#191485

Purpose

Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 S310F

Plasmid

#40991

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

S310F

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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TFORF0169

Plasmid

#141540

Purpose

Lentiviral vector for overexpressing the SIX3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SIX3 (SIX3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.hSyn.Cre.WPRE.hGH

Plasmid

#105553

Purpose

AAV expression of Cre from hSyn promoter

Depositor

James M. Wilson

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

Plasmid

#191484

Purpose

Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIB (w297-1)

Plasmid

#22800

Depositor

Eric Campeau

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pCIN BRCA2 WT

Plasmid

#16245

Depositor

Mien-Chie Hung

Article

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flpo

Plasmid

#55637

Purpose

Expresses Flpo in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde, AAV1, and AAV2

Insert

Flpo

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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