78,002 results

1. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

   Plasmid

   #129631

   Purpose

   Visualization of Akt and ERK activity

   Depositor

   Dorus Gadella

   Article

   Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

   Insert

   H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-HA-pcDNA3.1-

   Plasmid

   #52535

   Purpose

   enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCX-OKS-2A

   Plasmid

   #19771

   Purpose

   Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Science. 2008 Nov 7. 322(5903):949-53.

   Insert

   Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
   

   Expression

   Mammalian

   Mutation

   Three genes are connected with the foot-and-mouth…

   Available Since

   Jan. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CAG-dCasRx-RBM25-IRES-BleoR

   Plasmid

   #221002

   Purpose

   Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25-IRES-BleoR
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBbi-Bla

   Plasmid

   #60526

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. HDM-tat1b

   Plasmid

   #204154

   Purpose

   Lentivirus helper plasmid coding for Tat

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   tat
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TLR7-YFP

   Plasmid

   #13022

   Depositor

   Doug Golenbock

   Insert

   TLR7 (TLR7 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/HF-CDC50A

   Plasmid

   #209223

   Purpose

   Mammalian expression of CDC50A

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   CDC50A (TMEM30A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-ATR WT

    Plasmid

    #31611

    Depositor

    Aziz Sancar

    Article

    Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

    Insert

    ATR (ATR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lyso-GA

    Plasmid

    #209874

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Tags

    LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA GSK3 beta K85A pcDNA3

    Plasmid

    #14755

    Depositor

    Jim Woodgett

    Article

    Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

    Insert

    GSK3 beta K85A (GSK3B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K85A. Kinase dead mutant.

    Available Since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pmTurquoise2-T2A-Venus(L68V)

    Plasmid

    #60494

    Purpose

    Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2-T2A-mVenus(L68V)
    

    Tags

    T2A peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-mEGFP(A206K)-paCaMKII(K42M)

    Plasmid

    #165440

    Purpose

    Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)
    

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mRuby2

    Plasmid

    #40260

    Purpose

    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    mRuby2
    

    Tags

    His6 and Xpress

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKT2/CAGXSP/CD63mScarlet

    Plasmid

    #182972

    Purpose

    Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

    Insert

    CD63-mScarlet (CD63 Human)
    

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid

    #224898

    Purpose

    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

    Depositor

    Thomas Norman

    Article

    Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

    Inserts

    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTet-IRES-EGFP

    Plasmid

    #64238

    Purpose

    Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

    Depositor

    Maria Lung

    Article

    Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    pTet

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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