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pAAV-hSyn-fDIO-mCherry-WPREpA

Plasmid

#177328

Purpose

Control vector for Flp-dependent DREADD constructs

Depositor

Ulrik Gether

Insert

mCherry

Use

AAV; Flp-frt

Expression

Mammalian

Promoter

Human Synapsin-1

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0495

Plasmid

#143031

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EGR2 (EGR2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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VRQR-ABEmax

Plasmid

#119811

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHaloTag-EGFP

Plasmid

#86629

Purpose

Expresses HaloTag-eGFP tandem (cytosolic)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6f.WPRE.SV40

Plasmid

#100837

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG

Plasmid

#46956

Purpose

Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-hSYN-MYC-MIRO1

Plasmid

#219577

Purpose

Lentiviral construct expressing MYC-tagged human Miro1

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Miro1 (RHOT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Promoter

hSYN1

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_eGFP-P2A-luc2pre (3'UTR)

Plasmid

#240254

Purpose

Non-attenuated SFV vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

EGFP-P2A-firefly luciferase

Expression

Mammalian

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_TetR-VP16 (3'UTR)_RLEloop

Plasmid

#240259

Purpose

Attenuated PROTEUS vector with tTA transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

TetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTRE3G_G

Plasmid

#240261

Purpose

Circuit-responsive (TRE3G promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during tTA/rtTA-3G campaigns.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

VSV-G

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_TRE3G_luciferase

Plasmid

#240266

Purpose

Circuit-responsive (TRE3G promoter) expression of firefly luciferase. Used for evaluation of tTA/rtTA-3G transgene activity.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TRE3G

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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mCherry_ALFA_H2B

Plasmid

#244055

Purpose

anchoring mCherry-1x ALFAto the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

mCherry-ALFA-HIST1H2BJ

Tags

mCherry-ALFA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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bp::PhiC31

Plasmid

#140505

Purpose

Encodes PhiC31 recombinase downstream of a minimal promoter element

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

PhiC31

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEF-1α-Venus-Nup107-FRT

Plasmid

#247344

Purpose

Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

Nup107 (NUP107) (NUP107 Human)

Tags

Venus

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CAaNa::GFP

Plasmid

#140507

Purpose

Encodes GFP downstream of an attP/attB flanked Neomycin stop cassette driven by the CAG promoter

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

attP-Neomycin-PolyA-attB

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Endo-InPGrp

Plasmid

#236003

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Endo-InPGrp (Cyth3 Synthetic, Rat)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-PH-BTKx1

Plasmid

#183653

Purpose

PtdIns(3,4,5)P3 biosensor

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

BTK (BTK Frog, Human)

Tags

X. laevis map2k1.L(32-44)-mCherry

Expression

Mammalian

Mutation

Amino acids 2-170

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A24_STOP

Plasmid

#161146

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A24 (SLC25A24 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Stat3 Flag pRc/CMV

Plasmid

#8707

Depositor

Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

Stat3 (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

*see comments

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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