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80,589 results

Showing: 10481 - 10520 of 80589 results

  6. pTRIPZ-NUDT17-C-HA-Puro

    Plasmid
    #195182
    Purpose
    Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.
    Depositor
    Stephanie Roessler
    Insert
    NUDT17 (NUDT17 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV promoter and minimal CMV
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195182-plasmid-map-sequence-id-423138

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  8. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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  9. MultiMate-PE3 HEK3

    Plasmid
    #206273
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206273-plasmid-map-sequence-id-406645

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  16. pD649-HAsp-FCMR-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156684
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FCMR (FCMR Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156684-plasmid-map-sequence-id-504966

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  17. pSCMV-H2B-FT-Medium

    Plasmid
    #157670
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Medium-FT
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157670-plasmid-map-sequence-id-308113

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  21. tdTomato-TOMM20-N-10

    Plasmid
    #58137
    Purpose
    Localization: Outer Mitochondrial Membrane, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. PB-FLuc+GFPd2

    Plasmid
    #127190
    Purpose
    Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)
    Depositor
    Jordan Green
    Insert
    Firefly luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127190-plasmid-map-sequence-id-248023

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  33. Human a3 nicotinic receptor subunit EM construct

    Plasmid
    #163960
    Purpose
    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Asn348-Ser402 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available Since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163960-plasmid-map-sequence-id-323882

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  40. IKK1-EYFP

    Plasmid
    #111206
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    IKKalpha (CHUK Human)
    Tags
    EYFP
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111206-plasmid-map-sequence-id-212965

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Showing: 10481 - 10520 of 80589 results