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82,530 results

Showing: 6601 - 6620 of 82530 results

  3. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid
    #185910
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185910-plasmid-map-sequence-id-363739

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  7. PEA1-GFP

    Plasmid
    #171993
    Purpose
    Delivers all prime editing nickase components in a single, GFP selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171993-plasmid-map-sequence-id-340552

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  10. pCW57.1 Lysosomal-METRIQ

    Plasmid
    #135401
    Purpose
    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity
    Depositor
    Eisuke Itakura
    Publication
    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10
    Insert
    DNAse II alpha (DNASE2 Human)
    Use
    Lentiviral
    Tags
    T2A, mCherry, and sfGFP
    Expression
    Mammalian
    Mutation
    Single-nucleotide polymorphism C432T
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135401-plasmid-map-sequence-id-273129

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  12. pTRE3G-Bi-α-chain

    Plasmid
    #133730
    Purpose
    Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.
    Depositor
    Erin Schuman
    Publication
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    a-Chain_gePSI
    ZsGreen1
    Expression
    Mammalian
    Mutation
    Only one part of the gePSI-system (aa1-141 + stop…
    Promoter
    pTRE3G-Bi
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    133730-plasmid-map-sequence-id-266129

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  13. pCAGGs-RFP

    Plasmid
    #127347
    Purpose
    Expresses RFP constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Red Fluorescent Protein
    Expression
    Mammalian
    Promoter
    CAGGS Promoter
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127347-plasmid-map-sequence-id-246568

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Showing: 6601 - 6620 of 82530 results