78,292 results

1. GCaMP-AMPK-SPARK

   Plasmid

   #226257

   Purpose

   Dual reporter of Ca2+ levels and AMPK activity

   Depositor

   Roland Malli

   Article

   Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

   Insert

   GCaMP-AMPK-SPARK
   

   Expression

   Mammalian

   Available Since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. TFORF2165

   Plasmid

   #141953

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GRHL3 (GRHL3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hSyn-DIO-somBiPOLES-mCerulean

   Plasmid

   #154951

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF3132

   Plasmid

   #144608

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ESR2 (ESR2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF2744

   Plasmid

   #144225

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   T (TBXT Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Kv1.4 K+ channel (extracellular) scFv [L71/5]

   Plasmid

   #190517

   Purpose

   Mammalian Expression of Kv1.4 K+ channel (extracellular) scFV. Derived from hybridoma L71/5.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant scFV (Kcna4 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS_cAMPFIRE-L-R279E

   Plasmid

   #182286

   Purpose

   Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4/TO/GFPZNF598-9P-9A

   Plasmid

   #141192

   Purpose

   Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   ZNF598 (ZNF598 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   all 3 proline repeats are mutated to Alanine (9xP…

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF0983

   Plasmid

   #143739

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF5 (KLF5 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV2-SynTetOff-GFP

    Plasmid

    #190231

    Purpose

    AAV vector, high-level expression of EGFP in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-TRAM-CFP

    Plasmid

    #13027

    Depositor

    Doug Golenbock

    Insert

    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF3104

    Plasmid

    #144580

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX2-5 (NKX2-5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197026

    Purpose

    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Expression

    Mammalian

    Mutation

    D52R / T132R

    Promoter

    CBh, CMV, hU6

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid

    #9002

    Depositor

    William Sellers

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    T226A

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PTGER4-Tango

    Plasmid

    #66486

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    PTGER4 (PTGER4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4-TO-Hygromycin-sfGFP-MAP

    Plasmid

    #44100

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Tags

    sfGFP-MAP

    Expression

    Mammalian

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE mCherry-Rab11A

    Plasmid

    #228035

    Purpose

    Mammalian lentiviral expression of mCherry-labeled Rab11.

    Depositor

    Wade Harper

    Insert

    Rab11A (RAB11A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. A*11:01:01

    Plasmid

    #203281

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*11:01:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNAHygro(+)LAMP2B

    Plasmid

    #86029

    Purpose

    Expresses LAMP2B in Mammalian cells

    Depositor

    Janice Blum

    Article

    Zhou et al Immunity. 2005 May;22(5):571-81.

    Insert

    Lysosome-associated membrane protein 2b (LAMP2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. VPS13D^mScarlet

    Plasmid

    #174110

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    With an internal mScarlet tag in aminoacid positi…

    Promoter

    CMV

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge