75,901 results

1. pMP416

   Plasmid

   #134996

   Purpose

   dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
   

   Use

   Synthetic Biology

   Tags

   3xFLAG, E coli Dam(N132A), and dCas9

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rat Alpha2 delta-1 HA pMT2

   Plasmid

   #58729

   Purpose

   expression of HA tagged rat Alpha2 delta-1 in mamalian cells

   Depositor

   Annette Dolphin

   Article

   Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

   Insert

   alpha2 delta-1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGIP

   Plasmid

   #26777

   Purpose

   Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   EF1a-GFP-IRES-Puro (gfp )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

   Plasmid

   #118163

   Purpose

   CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lamin A (R377H)-mRFP

   Plasmid

   #124270

   Purpose

   Lamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R377H) (LMNA Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R377H)

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO/Strep-HA-ZAK alpha

   Plasmid

   #141193

   Purpose

   Expresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 (MAP3K20 Human)
   

   Use

   Tags

   Strep, HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR Lck-TagBFP

   Plasmid

   #171034

   Purpose

   TagBFP-tagged Lck kinase

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   Lck
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-NIR-GECO2

   Plasmid

   #159603

   Purpose

   AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

   Depositor

   Robert Campbell

   Article

   Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

   Insert

   NIR-GECO2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

   Plasmid

   #100842

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-trGluc

    Plasmid

    #158959

    Purpose

    DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    trGluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LeGO-C

    Plasmid

    #27348

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIS1 mLPIN1

    Plasmid

    #60803

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains LPIN1 3' UTR and mutated miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    LPIN1 3'UTR and mutated miR-155 binding site (LPIN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated miR-155 binding site

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFPN1-human Dynamin 1aa K44A

    Plasmid

    #22197

    Depositor

    Pietro De Camilli

    Article

    Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

    Insert

    human Dynamin 1aa K44A (DNM1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-SLC25A5_STOP

    Plasmid

    #161173

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A5 (SLC25A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. p2,0_3M5M_luciferase

    Plasmid

    #135475

    Purpose

    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, luciferase reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-ZDHHC5

    Plasmid

    #231654

    Purpose

    Expresses human ZDHHC5 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC5 (ZDHHC5 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PA-mCherry1-N1

    Plasmid

    #54507

    Purpose

    Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

19. p2xFLAGhYAP1-S127A

    Plasmid

    #17790

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein mutant S127A (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Serine 127 to Alanine

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. VPS13C^Halo-△(ATG2C-PH)

    Plasmid

    #232867

    Purpose

    Expresses VPS13C-△(ATG2C-PH) in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    VPS13C-△(ATG2C-PH) (VPS13C Human)
    

    Use

    Tags

    internal Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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