75,952 results

1. pHR-PGK-PYL1-sfGFP-Coilin

   Plasmid

   #122177

   Purpose

   expression of PYL1-SfGFP-Coilin, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   Coilin (COIL Human)
   

   Use

   Lentiviral

   Tags

   PYL1-SFGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. ADRA1A-Tango

   Plasmid

   #66213

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ADRA1A (ADRA1A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Flag HBx (G124L, I127A)

   Plasmid

   #42598

   Depositor

   Ding Xue

   Article

   Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

   Insert

   Hepatitis B virus protein X (G124L, I127A)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   G124L I127A

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pPalmitoyl-mTurquoise2

   Plasmid

   #36209

   Purpose

   In vivo visualization of the membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   p63(1-29) (ARHGEF25 Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. peGFP C2-IST1

   Plasmid

   #218582

   Purpose

   Expresses GFP-tagged human IST1 in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. haA1.pBABEpu

   Plasmid

   #21813

   Depositor

   David Ron

   Article

   Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

   Insert

   GADD34 C-term
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-GN

   Plasmid

   #60517

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiPGK Puro DEST ERKKTRClover

   Plasmid

   #90227

   Purpose

   Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-HA-Nrxn1beta AS4(-)

    Plasmid

    #59409

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF3137

    Plasmid

    #144613

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HEY1 (HEY1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis6-hDF

    Plasmid

    #72585

    Purpose

    human dysferlin full length tagged with His

    Depositor

    Renzhi Han

    Article

    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

14. 2Flag-pDEST-C

    Plasmid

    #118372

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TARDBP

    Plasmid

    #156073

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1 hPHGDH

    Plasmid

    #154916

    Purpose

    Doxycyclin inducible expression of human PHGDH

    Depositor

    Richard Possemato

    Article

    Richard et al Richard Possemato Lab plasmids

    Insert

    PHGDH (PHGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-FLAG-LOV*-H2B

    Plasmid

    #202204

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Histone H2B type 1-K
    

    Use

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TurboID-CRaf

    Plasmid

    #226168

    Purpose

    Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-CRaf (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-IRES-BlastR and Myc-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV500 empty vector

    Plasmid

    #33348

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Myc-βarrestin2-LOV-Turbo_pCDNA3

    Plasmid

    #199671

    Purpose

    expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Tags

    Myc and βarrestin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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