74,914 results

1. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MYC-hTRAK1

   Plasmid

   #225204

   Purpose

   Expresses MYC-tagged human TRAK1

   Depositor

   Thomas Schwarz

   Article

   Pekkurnaz et al Cell. 2014 Jul 3;158(1):54-68. doi: 10.1

   Insert

   trak1 (TRAK1 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE 3xHA-MKK6-EE puro

   Plasmid

   #216791

   Purpose

   Mammalian expression of constitutively active human MKK6

   Depositor

   Kevin Janes

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Retroviral

   Tags

   3x-HA

   Expression

   Mammalian

   Mutation

   S207E and T211E, constitutively active

   Promoter

   LTR

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC-C Sars-CoV-2 NSP12_P314L

   Plasmid

   #194813

   Purpose

   Sars-CoV-2 NSP12_P314L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP12_P314L (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P314L

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. UTS2R-DuET

   Plasmid

   #213386

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   UTS2R (UTS2R Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiT3.1-N_TLE1:1-770

   Plasmid

   #211248

   Purpose

   Mammalian expression of full-length human TLE1. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   TLE1:M1-Y770 (TLE1 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Rab13 in pCSDEST2

   Plasmid

   #140879

   Purpose

   Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab13 (rab13 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. NDV-HN-SpyTag

   Plasmid

   #160000

   Purpose

   Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   NDV-HN-SpyTag
   

   Use

   Tags

   His6-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta-actin promoter

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-puro-NKX2-8

   Plasmid

   #75080

   Purpose

   retroviral vector holding human NKX2-8 cDNA

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Insert

   NKX2-8 (NKX2-8 Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild-type, full-length, without native 3' UTR

   Promoter

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mTagBFP2

    Plasmid

    #122373

    Purpose

    Expresses mTagBFP2 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Promoter

    Available since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. scaffold-linker-U6

    Plasmid

    #89639

    Purpose

    construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Use

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX313-Firefly luciferase

    Plasmid

    #118017

    Purpose

    Constitutive expression of Firefly luciferase

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid

    #26429

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3_cAMPFIRE-M

    Plasmid

    #182280

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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