78,130 results

1. pEF-XMAS-1xStop

   Plasmid

   #164411

   Purpose

   Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-GFP

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-FRT/TO-3xFLAG-REV7

   Plasmid

   #114127

   Purpose

   Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV/TetO2

   Available Since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TFORF0209

   Plasmid

   #141556

   Purpose

   Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXP2 (FOXP2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-mBAF45b IRES GFP

   Plasmid

   #17881

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45b (Dpf1 Mouse)
   

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Available Since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pLNCX2-CFP-VGPDFGR-YFP

   Plasmid

   #59587

   Purpose

   Encodes a granzyme B FRET reporter

   Depositor

   Tim Mitchison

   Article

   Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

   Insert

   ECFP-VGPDFGR-Venus
   

   Use

   Retroviral

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PresentER-SIINFEKL (GFP)

   Plasmid

   #102944

   Purpose

   PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

   Depositor

   David Scheinberg

   Article

   Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

   Insert

   SIINFEKL (OVAL Chicken, Synthetic)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   OVA257-264

   Promoter

   LTR

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FCK-Arch-GFP

   Plasmid

   #22217

   Purpose

   Lentiviral expression of Arch-GFP for optogenetic inhibition

   Depositor

   Edward Boyden

   Article

   Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

   Insert

   Arch
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Jan. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF3095

   Plasmid

   #144571

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LIN9 (LIN9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1920

   Plasmid

   #142887

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RUNX1 (RUNX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL20_mEGFP_RUNX1_RUNX1T1

    Plasmid

    #205915

    Purpose

    Express mEGFP-tagged fusion protein, RUNX1_RUNX1T1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    RUNX1_RUNX1T1 (RUNX1, RUNX1T1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP STAT1 Y701F

    Plasmid

    #12302

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

    Insert

    STAT1 Y701F (STAT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Y701F

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

12. CRY2(L348F)-CreN

    Plasmid

    #75368

    Purpose

    Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    Cre recombinase, residues 19-104

    Expression

    Mammalian

    Mutation

    L348F

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSNAP-CLC-SNAP

    Plasmid

    #38012

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    SNAP26m

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-hGLIS1

    Plasmid

    #37625

    Purpose

    Non-integrating (episomal) expression of human GLIS1

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    human GLIS1 (GLIS1 Human)
    

    Expression

    Mammalian

    Mutation

    T110A, A187G, I221T

    Promoter

    CAG

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT374

    Plasmid

    #125615

    Purpose

    Expresses evoFERNY-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoFERNY-BE4max-NG
    

    Expression

    Mammalian

    Mutation

    H102P D104N

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid

    #200904

    Purpose

    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGFP-ATIC

    Plasmid

    #99110

    Purpose

    Expresses human GFP-ATIC fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    ATIC (ATIC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S65T mutation in GFP gene

    Promoter

    CMV

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3HA-hMIB1

    Plasmid

    #33317

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB1 (MIB1 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. 7b sgRNA for EJ7-GFP reporter

    Plasmid

    #113624

    Purpose

    sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7b sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pGL3-cMYC promoter

    Plasmid

    #114724

    Purpose

    reporter luciferase construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Oncogene. 1994 Feb;9(2):405-15.

    Insert

    C-Myc promoter region
    

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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