74,946 results

1. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

   Plasmid

   #229787

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; ZF10-NS3-p65
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

   Plasmid

   #204146

   Purpose

   Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Inserts

   SARS-CoV-2 BA.1 variant spike
   zsGreen-T2A-PuR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   21 amino acid cytoplasmic tail deletion

   Promoter

   CMV and TRE3G

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNEU-hMbPylRS-4xU6M15

   Plasmid

   #105830

   Purpose

   Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

   Depositor

   Irene Coin

   Article

   Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

   Inserts

   Humanized MbPylRS
   tRNAM15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y271A, Y349F)MbPylRS

   Promoter

   CMV and U6

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Solo-mStayGold-TJP1

   Plasmid

   #227300

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-TJP1

   Plasmid

   #227299

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-AID-BLM-UGI-T2A-mTagBFP2

   Plasmid

   #221222

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-BLM-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-NLS-HA-TurboID

   Plasmid

   #215075

   Purpose

   Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   TurboID (birA )
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLXRN-EGFP

   Plasmid

   #99207

   Purpose

   EGFP expressing bicistronic retroviral vector

   Depositor

   Peter Pedersen

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A Kozak sequence was incorporated to enhance tran…

   Promoter

   LTR

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-GCaMP6s-WPRE-sv40

    Plasmid

    #184414

    Purpose

    AAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoter

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLYBL-TO-hNGN2-BSD-mApple

    Plasmid

    #124229

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-R-iLACCO1.1

    Plasmid

    #208026

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Htr5b(3.7)-tTA-sv40

    Plasmid

    #184412

    Purpose

    AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-tTA
    

    Use

    AAV

    Tags

    tTA

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

    Plasmid

    #187576

    Purpose

    Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    LMNA (LMNA Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nsp12-EGFP

    Plasmid

    #165116

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp12 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC13N-dCas9-BFP-KRAB

    Plasmid

    #127968

    Purpose

    constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-dCas9-NLS-BFP-KRAB
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-sapphireCKAR

    Plasmid

    #118468

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Use

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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