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pEGFP-C1-ADRP

Plasmid

#87161

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

Plasmid

#60226

Purpose

Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Renilla luciferase
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

Tags

Cre-HA and Rluc-P2A

Expression

Mammalian

Promoter

EFS and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1

Plasmid

#36412

Depositor

Benjamin Glick

Type

Empty backbone

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pT3-N90-beta-catenin

Plasmid

#31785

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of aa 1-90- Constitutitvely Active

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pSlow-FT-N1

Plasmid

#31912

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA2

Plasmid

#26678

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 2 (KPNA2 Human)

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO-p53-shRNA-427

Plasmid

#25636

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-427 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-CUL5

Plasmid

#19895

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 5 (CUL5 Human)

Tags

myc3

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5 beta1

Plasmid

#16042

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

beta1 (ITGB1 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro p53 shRNA 2

Plasmid

#10672

Depositor

William Hahn

Article

Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

Insert

p53 shRNA (TP53 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pWZL Blast myc

Plasmid

#10674

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

c-myc (MYC Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-MmPeg10rc4

Plasmid

#174858

Purpose

Expresses recoded mouse Peg10 in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

recoded Mus musculus PEG10 (Peg10 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

Plasmid

#101139

Purpose

Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pN1 Lck-GCaMP5G

Plasmid

#34924

Purpose

Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

Depositor

Baljit Khakh

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

Lck-GCaMP5G

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro empty (w214-1)

Plasmid

#17484

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-cMYC

Plasmid

#20324

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

cMYC (Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pDESTmycDICER

Plasmid

#19873

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

dicer 1, ribonuclease type III (DICER1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe-c-myc-zeo

Plasmid

#17758

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

c-myc (MYC Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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