74,946 results

1. EF.hICN1.Ubc.GFP

   Plasmid

   #17626

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   ICN1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-SUN1_916

   Plasmid

   #125850

   Purpose

   Expresses SUN1_916 tagged with GFP in mammalian cells

   Depositor

   Miki Hieda

   Article

   Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

   Insert

   SUN1_916 (SUN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   stop codon

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor

   Plasmid

   #229788

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; ZF10-NS3-p65
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ZF10 and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-neo FlucDM EGFP

   Plasmid

   #90172

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R188Q, R261Q in FLuc

   Promoter

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

   Plasmid

   #187575

   Purpose

   Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SRSF1 (SRSF1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV_SARS-CoV-2_Sdel18

   Plasmid

   #207307

   Purpose

   Mammalian expression of SARS2 coronavirus S protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SARS-CoV2_S (S SARS2 coronavirus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pflareG-psd95-exon18

   Plasmid

   #90266

   Purpose

   Minigene reporter for alternative splicing analysis

   Depositor

   Douglas Black

   Article

   Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

   Insert

   Psd95-exon18 (Dlg4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

   Plasmid

   #229783

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   LacZ; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-GA_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229785

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2T-CMV-EA-BE4max-NG-BlastR

    Plasmid

    #152992

    Purpose

    High purity C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    BE4 H47E+S48A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHCMV_HCV_E1E2

    Plasmid

    #207279

    Purpose

    Mammalian expression of Hepatitis C virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    HCV_E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. RFP Dynamin2 Wild Type

    Plasmid

    #128152

    Purpose

    Expression of Dynamin 2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

    Insert

    Dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LDB-3-p65

    Plasmid

    #158984

    Purpose

    Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    LDB-3-p65
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Thy1.1-GFP

    Plasmid

    #131703

    Purpose

    encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GPR65-Tango

    Plasmid

    #66370

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR65 (GPR65 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid

    #123235

    Purpose

    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1(Hygro) myc-hPolQ-Flag

    Plasmid

    #73132

    Purpose

    To express in mammalian cells human PolQ

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. tdTomato-PDHA1-N-10

    Plasmid

    #58124

    Purpose

    Localization: Mitochondria, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    PDHA1 (PDHA1 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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