1,782 results

Showing: 361 - 390 of 1782 results

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pJH1579

Plasmid

#178904

Purpose

Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

YC3.60

Expression

Worm

Mutation

See Depositor Comments Below

Promoter

Pglr-1

Available Since

Jan. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pSM08

Plasmid

#139042

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mscarlet (includes STOP codon)

Expression

Worm

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159850

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMS050

Plasmid

#91826

Purpose

Codon-optimized mScarlet-I donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mScarlet-I-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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SP6-sgRNA-scaffold

Plasmid

#47912

Purpose

Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

Depositor

Paul Sternberg

Article

Chiu et al Genetics. 2013 Aug 26.

Type

Empty backbone

Use

CRISPR

Tags

sgRNA 3' end

Expression

Worm

Promoter

SP6

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pJW1816

Plasmid

#154320

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1::ChR2(H134R)::mCherry

Plasmid

#117420

Purpose

For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

channel rhodopsin-2 with H134R mutation

Expression

Worm

Mutation

H134R

Promoter

inx-1

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ1662

Plasmid

#51482

Purpose

miniMos transposable element with HygroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pJA304

Plasmid

#28091

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR

Expression

Worm

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pNM3724

Plasmid

#154112

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEG345

Plasmid

#40077

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (mex-5 Nematode)

Tags

Dendra2 PFAF, S-peptide, and TEV protease cleavag…

Expression

Worm

Promoter

pie-1

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pJH4723

Plasmid

#178899

Purpose

Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pges-1

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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3xHA-BioID_pAS28

Plasmid

#118223

Purpose

expresses 3xHA-tagged BioID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

BioID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

R118G

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJH1371

Plasmid

#199631

Purpose

Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GPI

Tags

YFP

Expression

Worm

Promoter

Pregf-1

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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punc-119c

Plasmid

#21595

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119 (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Expression

Worm

Mutation

N/A

Available Since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pJF6

Plasmid

#139040

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

gfp (includes STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1839

Plasmid

#154325

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ210

Plasmid

#30538

Depositor

Erik Jorgensen

Insert

ttTi4348 region

Expression

Worm

Available Since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDD378

Plasmid

#91827

Purpose

Codon-optimized HaloTag donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

HaloTag-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

Plasmid

#193852

Purpose

Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

Guide RNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1347

Plasmid

#154310

Purpose

Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa Linker for CT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

Plasmid

#225924

Purpose

Expressing LGC-47 in AVA neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

rig-3

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKS235

Plasmid

#191855

Purpose

Negative selection marker for MosSCI

Depositor

K Subramaniam

Article

Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

Insert

peel-1 toxic protein for negative selection against extrachromosomal array

Expression

Worm

Promoter

hsp-16.41

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Pvha6-AgDD

Plasmid

#80626

Purpose

create protein aggregates in C. elegans

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Worm

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pPD95_25

Plasmid

#1486

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, ATG-SecSig, pes10

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pNM3732

Plasmid

#153086

Purpose

mec-4p GFP-C1 positive control for RMCE integration

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p GFP-C1 tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW2341

Plasmid

#186950

Purpose

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Depositor

Jordan Ward

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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1,782 results