1,779 results

1. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159814

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD121

   Plasmid

   #91833

   Purpose

   Cas9 expression in C. elegans

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. L4817

   Plasmid

   #1683

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   myo3-2341, myo3Tsa, gfpN, IVSR, gfpGLA, IVSS, IVS…

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRK138

   Plasmid

   #173746

   Purpose

   CeLINC plasmid

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   Prps-0::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
   

   Expression

   Worm

   Promoter

   Prps-0

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD364

   Plasmid

   #91831

   Purpose

   Lox511I-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox511I-SEC-Lox511I
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH4203

   Plasmid

   #200160

   Purpose

   Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   twk-40 (twk-40 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Prig-3

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDD382

   Plasmid

   #91830

   Purpose

   Lox2272-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox2272-SEC-Lox2272
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNM3724

   Plasmid

   #154112

   Purpose

   tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   tetO 7X::∆pes-10::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJR81

   Plasmid

   #75029

   Purpose

   tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Insert

   tagBFP2^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized tagBFP2

   Expression

   Worm

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PU6::klp-12_sgRNA

    Plasmid

    #46170

    Purpose

    klp-12 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    klp-12 targeting sgRNA (klp-12 Synthetic)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available Since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBN396

    Plasmid

    #109326

    Purpose

    Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

    Depositor

    Peter Askjaer

    Article

    Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

    Insert

    mex-5 (mex-5 Nematode)
    

    Expression

    Worm

    Promoter

    mex-5

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

    Plasmid

    #182351

    Purpose

    Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

    Plasmid

    #66753

    Purpose

    miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

    Depositor

    Yishi Jin

    Article

    Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    miniSOG
    

    Tags

    tomm-20 (N terminal 55 aa)

    Expression

    Worm

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJR021

    Plasmid

    #198814

    Purpose

    Variant of yellow fluorescent protein for transient gene expression

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::destabilizedYFP::let-838 3'UTR
    

    Expression

    Worm

    Mutation

    YFP with destabilized signal from mouse ornithine…

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHW450

    Plasmid

    #198831

    Purpose

    Voltage-sensitive fluorescent protein.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1839

    Plasmid

    #154325

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJF6

    Plasmid

    #139040

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    gfp (includes STOP codon, PATC introns)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD378

    Plasmid

    #91827

    Purpose

    Codon-optimized HaloTag donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    HaloTag-C1
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159813

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRB1080

    Plasmid

    #71309

    Purpose

    Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

    Depositor

    Andrew Fire

    Article

    Bell et al Genetics. 2015 Dec 17. pii: genetics.115

    Insert

    VQR Cas9 variant
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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