1,755 results

1. pAS1-lim-4P::his-24::Cerulean

   Plasmid

   #124336

   Purpose

   Multicolor Worms for Neuronal ID

   Depositor

   Andrew Leifer

   Insert

   lim-4, Cerulean (lim-4 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. XW09

   Plasmid

   #65833

   Purpose

   Punc-4c-QS-SL2-mcherry3'UTR-unc-54

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   Neurospora crassa OR74A repressor (qa-1S)
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   Promoter

   Punc-4c

   Available since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHygCG2r

   Plasmid

   #200074

   Purpose

   hygR selection mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMS050

   Plasmid

   #91826

   Purpose

   Codon-optimized mScarlet-I donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   mScarlet-I-C1
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMB70

   Plasmid

   #47943

   Purpose

   Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS716

   Plasmid

   #188888

   Purpose

   Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   floxed Cbr-unc-119 marker and Phsp16.41::Cre
   

   Use

   Tags

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH3626

   Plasmid

   #179630

   Purpose

   Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2(s)

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJA281

   Plasmid

   #22513

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   Pmex-5::mCherry (w introns w/o stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHW581

   Plasmid

   #198818

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGC185

    Plasmid

    #19624

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ150-set2

    Plasmid

    #82546

    Purpose

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pvha6-AgDD

    Plasmid

    #80626

    Purpose

    create protein aggregates in C. elegans

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

    Plasmid

    #182358

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Pmex-5

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

    Plasmid

    #107133

    Purpose

    cGAL-N driver under the control of C. elegans hsp-16.41 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans hsp-16.41 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN444

    Plasmid

    #108331

    Purpose

    Expresses Sat2 for Nourseothricin resistance selection of C. elegans

    Depositor

    Shinsuke Niwa

    Article

    Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

    Insert

    Streptothricin acetyl transferase 2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-27 promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

    Plasmid

    #159799

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBN41

    Plasmid

    #86716

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-2::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 3xHA-BioID_pAS28

    Plasmid

    #118223

    Purpose

    expresses 3xHA-tagged BioID in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    BioID (BirA mutant)
    

    Use

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    R118G

    Promoter

    ges-1p

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEY51

    Plasmid

    #191054

    Purpose

    mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    mec-3 (mec-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDD288

    Plasmid

    #137017

    Purpose

    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    mNG-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge