8,135 results

1. pGBKT7-GW

   Plasmid

   #61703

   Purpose

   Gateway compatible Y2H vector - BD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGADT7-GW

   Plasmid

   #61702

   Purpose

   Gateway compatible Y2H vector - AD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLUM

   Plasmid

   #40276

   Depositor

   Markus Ralser

   Article

   Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

   Inserts

   LEU2 (LEU2 Budding Yeast)
   URA3 (URA3 Budding Yeast)
   MET15/17 (MET17 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   endogenous LEU2, endogenous MET15/17, and endogen…

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetR in pENTR1A (559-1)

   Plasmid

   #22265

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor (tetR )
   

   Expression

   Insect, Mammalian, Worm Exp…

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHES553 [prGal1-RAS2(KAN) pnh604]

   Plasmid

   #103846

   Purpose

   Yeast Expression Plasmid

   Depositor

   Hana El-Samad

   Article

   Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

   Insert

   pGal1-Ras2(KAN)
   

   Expression

   Yeast

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52 and pTDH3

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIV151

   Plasmid

   #226180

   Purpose

   Expresses Cas9 in a backbone compatible with K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   GAP1

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIV272

    Plasmid

    #226183

    Purpose

    Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    TEF1p (Komagataella phaffii)

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYES2.1-TRP1

    Plasmid

    #11007

    Depositor

    Francisco Malagon

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Tags

    V5 and his6

    Expression

    Bacterial and Yeast

    Mutation

    ORF-TRP1 without stop codon

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lifeact-3xmNG::URA3

    Plasmid

    #227595

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::URA3
    

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEK39

    Plasmid

    #210599

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
    

    Tags

    mRuby3

    Expression

    Bacterial and Yeast

    Promoter

    SpH2Bpro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGI3EM31

    Plasmid

    #135491

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCerulean3
    

    Tags

    mCerulean3

    Expression

    Bacterial and Yeast

    Promoter

    SpunH2A/Bpr (divergent)

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYES2-HTH

    Plasmid

    #86470

    Purpose

    Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter

    Depositor

    Akash Gunjan

    Article

    Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

    Type

    Empty backbone

    Tags

    HIS10-TEV-HA

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. LHZ1494

    Plasmid

    #222104

    Purpose

    Triple sgRNAs expression plasmid in Kluyveromyces marxianus.

    Depositor

    Yongming Wang

    Insert

    sg1RNA, sg4RNA, sg7RNA
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LHZ1493

    Plasmid

    #222102

    Purpose

    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCTcon2

    Plasmid

    #41843

    Depositor

    Dane Wittrup

    Article

    Chao et al Nat Protoc. 2006;1(2):755-68.

    Insert

    CD20
    

    Tags

    HA and c-myc

    Expression

    Yeast

    Available Since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB5320

    Plasmid

    #204830

    Purpose

    An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Expression

    Yeast

    Promoter

    CMV promoter and enotetSW2 promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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