8,086 results

1. pVS1

   Plasmid

   #33143

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Insert

   LacO (256 copies)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

2. ZJOM135

   Plasmid

   #133659

   Purpose

   Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS416-ADH1p-TID-FKBP-HA3

   Plasmid

   #227998

   Purpose

   Expresses TurboID-FKBP fusion from ADH1 promoter; yeast BioID

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FKBP
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBKT7-GW

   Plasmid

   #61703

   Purpose

   Gateway compatible Y2H vector - BD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDDGFP_LEU2D

   Plasmid

   #58352

   Purpose

   Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

   Depositor

   Simon Newstead

   Article

   Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   GFP-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCYC1m_yeGFP

   Plasmid

   #64389

   Purpose

   encodes yeast enhanced GFP under control of minimal pCYC1 promoter

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pCYC1

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSJ95

   Plasmid

   #104343

   Purpose

   Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   FBP1
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-6xGLY-3xFLAG-kanMX6

   Plasmid

   #20754

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-6xGLY-3xFLAG-hphMX4

   Plasmid

   #20755

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-6xGLY-His-tag-hphMX4

    Plasmid

    #20764

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-6xGLY-His-tag-kanMX6

    Plasmid

    #20763

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBD-pZ-FLAG

    Plasmid

    #183148

    Purpose

    Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 BD
    

    Use

    Yeast two-hybrid

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACT-Lex

    Plasmid

    #183149

    Purpose

    Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 AD
    

    Use

    Yeat two-hybrid

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPICZalphaB-SapL3

    Plasmid

    #78171

    Purpose

    Expression of Saporin L3 in pichia

    Depositor

    Vern Schramm

    Article

    Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

    Insert

    Saporin L3
    

    Use

    Tags

    alpha factor

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAPZa-STE2-1D4

    Plasmid

    #133422

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG426-GAL-Alpha-synuclein

    Plasmid

    #181715

    Purpose

    Galactose inducible expression of Alpha-synuclein

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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