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pNB2176

Plasmid

#113395

Purpose

Ty3-IN-GFP; alpha factor inducible

Depositor

Suzanne Sandmeyer

Article

Beliakova-Bethell et al RNA. 2006 Jan;12(1):94-101. doi: 10.1261

Insert

Ty3 with integrase-green fluorescent protein

Use

Tags

IN-GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBF3038

Plasmid

#26850

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2010 Sep 10. ():.

Insert

CreA recombinase

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

N/A

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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Cas9-NAT

Plasmid

#64329

Purpose

Cas9 expression cassette carried by a yeast single-copy episome plasmid

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB2904(XI-3 MarkerFree)

Plasmid

#73276

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pPM28 (eroGFP CEN/ARS URA3)

Plasmid

#20131

Depositor

Feroz Papa

Article

Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Insert

eroGFP (KAR2 jellyfish, Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pVTU-Dest

Plasmid

#105088

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Article

Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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p414-TEF1p-Cas9-CYC1t

Plasmid

#43802

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAV116

Plasmid

#63183

Purpose

pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2909(XII-5 MarkerFree)

Plasmid

#73281

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDB5320

Plasmid

#204830

Purpose

An integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

Depositor

Li-Lin Du

Article

Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

Inserts

tetR
mECitrine

Use

Tags

Expression

Yeast

Mutation

Promoter

CMV promoter and enotetSW2 promoter

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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BRR2 pRS313

Plasmid

#111411

Purpose

Brr2 (endogenous promoter)

Depositor

Christine Guthrie

Article

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pG4

Plasmid

#162605

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

tetO7 promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSC5GGv1

Plasmid

#194645

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pRPR1(TetO)

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJT130_GalL_nCDA1-NGBE3

Plasmid

#145094

Purpose

Expressing base editor nCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-NGBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRS306-OsTIR1F74A

Plasmid

#198416

Purpose

Expresses OsTIR1F74A under the control of ADH1 promoter in budding yeast

Depositor

Takumi Kamura

Article

Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

Insert

OsTIR1F74A

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1 promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomRuby2-Kan

Plasmid

#44953

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum

Plasmid

#20030

Depositor

Harmit Malik

Article

Elde et al Nature. 2008 Nov 30. ():.

Insert

Protein kinase R (EIF2AK2 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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