8,350 results
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Plasmid#227982PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertminiTurboID
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
NM9-SpCas9-NLS3
Plasmid#128177PurposeExpresses R. toruloides codon-optimized SpCas9 under PGK1 promoterDepositorInsertSpCas9-NLS3
UseCRISPRExpressionYeastPromoterpPGK1Available SinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPRa_VPR_yl
Plasmid#107677PurposeCRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-VPR
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYEXNHG3
Plasmid#49025PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastPromoterGAL1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:hTRAAK(W262S)
Plasmid#130670PurposeP. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFPDepositorInsertKCNK4 (KCNK4 Human)
TagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationN104Q, N108Q, W262SAvailable SinceApril 24, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BASU-HA3-KanMX6
Plasmid#227978PurposeTo insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertBASU
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BioID2-HA3-KanMX6
Plasmid#227976PurposeTo insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertBioID2
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BirA-HA3-KanMX6
Plasmid#227974PurposeTo insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 markerDepositorInsertBirA
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEmpty_Control
Plasmid#162607PurposeEmpty control for Cas9 experiments and to clone new guide RNAs for paperDepositorInsertTef1-Cas9 with RPL25 Intron
ExpressionYeastAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-NatMX6
Plasmid#229231PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS426GFP-2×PH(PLCδ)
Plasmid#36092DepositorInsert2xPH(PLCdelta) fusion
TagsGFPExpressionYeastMutationcontains aa11-140 of PLCδ (including the PH domai…PromoterPRC1Available SinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRR-MR-5Z
Plasmid#23059DepositorAvailable SinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pCAP03-acc(3)IV
Plasmid#69862PurposepCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceNov. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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YIplac211-SEC7-msCFPx6
Plasmid#105268PurposePlasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.DepositorAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIW601-KmCRISPR
Plasmid#98907PurposeK. marxianus CRISPR Plasmid for sgRNA cloningDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only