8,173 results
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Plasmid#44899PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selectionDepositorInsertyomTagBFP2
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastPromoternoAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEK39
Plasmid#210599PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
TagsmRuby3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_AXP_hrGFP
Plasmid#84613PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locusDepositorInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_haPD-1
Plasmid#226716PurposePlasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)DepositorInsertHigh affinity PD-1 microbody
TagsHA tag and Mating factor alpha secretion signalExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CRU313
Plasmid#131163PurposeFor ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA CUP1p-NuiHA kanMX
Plasmid#131169PurposePCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable SinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_XPR2_hrGFP
Plasmid#84614PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locusDepositorInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHES553 [prGal1-RAS2(KAN) pnh604]
Plasmid#103846PurposeYeast Expression PlasmidDepositorInsertpGal1-Ras2(KAN)
ExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1393
Plasmid#111430PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCRISPRyl_XPR2
Plasmid#84609PurposeIntroduces DSB at XPR2 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS156
Plasmid#182707PurposeCRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
ExpressionYeastAvailable SinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymNeongreen-T2A-mCherry
Plasmid#125700PurposeProduces equimolar expression of yeast codon optimized mNeongreen and mCherryDepositorInsertymNeongreen-T2A-mCherry
ExpressionYeastAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pML104-KanMx4
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
ExpressionYeastAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA Ser promoterAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM31
Plasmid#135491PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3DepositorInserthph SpunH2A/Bpr hph-mCerulean3
TagsmCerulean3ExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only