8,431 results
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Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorPublicationTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pCAP05
Plasmid#102920PurposeDirect cloning and broad-host-range heterologous expression vectorDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#102951PurposeExpresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoterDepositorInsertAPEX2-NES
TagsFLAGExpressionYeastPromoterGAL1Available sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWS175
Plasmid#90962PurposeCas9 gap repair vector - HygRDepositorInsertCas9
UseCRISPRExpressionYeastAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p403-BrdU-Inc
Plasmid#71789PurposeBrdU-incorporating plasmid with selectable marker HIS3DepositorAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAG426GPD-ccdB-YFP-yeastSMASh
Plasmid#68855PurposeExpresses SMASh tag fused to YFP at C terminus in yeastDepositorInsertYFP-yeastSMASh
TagsSMASh tagExpressionYeastPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBIS-GALkFLP(URA)
Plasmid#49460Purposeoverexpression of Flp step-arrest mutant in yeast, URA selectionDepositorPublicationInsertFlp(H305L) (flp Budding Yeast)
ExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterGAL10Available sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-FLAG-hphMX4
Plasmid#20752DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Myc-kanMX6
Plasmid#20769DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAG424GPD-ccdB
Plasmid#14152DepositorPublicationTypeEmpty backboneUseGateway CloningExpressionYeastAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-ymScarletI-URA3
Plasmid#168055PurposeReplaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymScarletI
TagsymScarletIExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWS174
Plasmid#90961PurposeCas9 gap repair vector - NatRDepositorInsertCas9
UseCRISPRExpressionYeastAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Human, Synthetic, Budding Yeast)
UseSynthetic BiologyExpressionYeastPromoterADH1 (crippled)Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGKB
Plasmid#85077Purposeexpression in Pichia pastorisDepositorPublicationTypeEmpty backboneExpressionYeastPromoterGAPAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPRyl_AXP
Plasmid#84608PurposeIntroduces DSB at AXP locus in Y. lipolyticaDepositorPublicationInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML104-NatMx3
Plasmid#83477PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.DepositorInsertNatMx3
ExpressionYeastAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p415 TEF roGFP2‐Tsa2ΔCPΔCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
TagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PhyB-mCherry-CAAX
Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-yETSapphire-CaUra3
Plasmid#44650DepositorInsertTurbo Sapphire
ExpressionYeastAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only