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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRS415-GPD

Plasmid

#183940

Purpose

Control vector that has a GPD promoter with no insert

Depositor

Michael Downey

Article

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Type

Empty backbone

Expression

Yeast

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

Plasmid

#117661

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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BB3cK_pGAP_23*_pLAT1_Cas9

Plasmid

#104908

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#162606

Purpose

Pre-express Cas9 in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Article

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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LHZ1493

Plasmid

#222102

Purpose

Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

Depositor

Yongming Wang

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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ZJOM68

Plasmid

#133657

Purpose

Integration of TGL3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-TGL3-mCherry (TGL3 Budding Yeast)

Tags

mCherry

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pYX233-b2-DHFR

Plasmid

#163759

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2-DHFR (Dhfr Budding Yeast, Mouse)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCM184-ParB2::eGFP

Plasmid

#87252

Purpose

Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB2

Tags

EGFP

Expression

Yeast

Promoter

pBAD/tetO

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pH3mCHSH2

Plasmid

#101053

Purpose

This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

Depositor

Jennifer Lodge

Article

Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

Insert

Cryptococcus mCherry expression plasmid

Expression

Yeast

Promoter

Cryptococcus Histone3 promoter

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#83946

Purpose

Cas9-carrying vector with kanMX marker

Depositor

Irina Borodina

Article

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

Cas9

Expression

Yeast

Available Since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-ALFA-HIS3MX6

Plasmid

#247627

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

ALFA

Expression

Yeast

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-HSE-YFP

Plasmid

#172131

Purpose

Fluorescent reporter for Hsf1 activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xHSE-YFP

Use

Vector for genomic integration into the yeast gen…

Expression

Yeast

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pYX233-b2delta-DHFR

Plasmid

#163760

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2delta-DHFR (Dhfr Budding Yeast, Mouse)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Expression

Yeast

Promoter

Actine

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCR

Plasmid

#83238

Purpose

The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCr

Tags

roGFP2 fusion with Tsa2dCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ccdB

Plasmid

#14150

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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