8,085 results

1. pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

   Plasmid

   #86417

   Purpose

   luminal split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-mCherry-SCS2TM-GFP11
   

   Use

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH2971

   Plasmid

   #100955

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFG2

   Plasmid

   #87250

   Purpose

   gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   Nat
   

   Use

   Tags

   parS segments

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

   Plasmid

   #86414

   Purpose

   nuclear split GFP11 reporter without mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-PUS1
   

   Use

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   NOP1pr

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAP05

   Plasmid

   #102920

   Purpose

   Direct cloning and broad-host-range heterologous expression vector

   Depositor

   Bradley Moore

   Article

   Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p405MET25

   Plasmid

   #15961

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   MET25 promoter is between SacI and XbaI restricti…

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEV-G4-Km

   Plasmid

   #46819

   Purpose

   TEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformation

   Depositor

   Claudia Vickers, Lars Nielsen

   Article

   Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

   Type

   Empty backbone

   Use

   Tags

   FLAG and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 and HXT7

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

   Plasmid

   #86421

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-KAR2SS-GFP1-10
   

   Use

   Tags

   GFP1-10

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS402-nicastrin/APH-1a-HA

    Plasmid

    #207804

    Purpose

    Yeast integrative vector for expression of Nicastrin and APH-1a-HA

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Gamma-secretase subunits nicastrin and Aph1a
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pESC-URA (V5 and HA)

    Plasmid

    #222485

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJA181

    Plasmid

    #128539

    Purpose

    Expresses anti-Prunus dulcis scFv Ab+BirA tag

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6 plus BirA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJA179

    Plasmid

    #128537

    Purpose

    Expresses anti-Prunus dulcis scFv Ab

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GalL

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBEVY-U

    Plasmid

    #51230

    Purpose

    bi-directional expression vector for yeast, constitutively active, URA3 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_XPR2_hrGFP

    Plasmid

    #84614

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT040

    Plasmid

    #67640

    Purpose

    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Insert

    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p405ADH1

    Plasmid

    #15966

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    p405ADH1 (ADH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    ADH1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPRyl_XPR2

    Plasmid

    #84609

    Purpose

    Introduces DSB at XPR2 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge