8,173 results
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Plasmid#141249PurposeGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterGAL1 (Cas9), pSNR52 (gRNA)Available SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only
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pDRF1 ymBaojin-T2A-mCherry
Plasmid#219857Purposeequimolar expression of ymBaojin and mCherry in budding yeastDepositorInsertymBaojin-T2A-mCherry
ExpressionYeastAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGY416
Plasmid#166660PurposeFor light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeastDepositorInsertLiCre
UseCre/LoxExpressionYeastMutationCre: E340A, D341APromoterGAL1Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec633
Plasmid#130873PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRepDepositorInsertTP-DNAP1
ExpressionYeastMutationL477V, L640Y, I777K, W814NAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyExpressionYeastAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gal:mCherry-uTEV3
Plasmid#135453PurposeYeast expression of uTEV3 fused to mCherryDepositorInsertmCherry-uTEv3
TagsmCherryExpressionYeastMutationS219V mutation improves stability , I138T/S153N/T…PromoterGalAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
ymScarlet
Plasmid#111917Purposeyeast optimized mScarletDepositorInsertymScarlet
ExpressionYeastPromoterGPDAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9
Plasmid#46920PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoterDepositorInsertdCas9
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-3xFLAG-hphMX4
Plasmid#20755DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSFS2A-CaHygB
Plasmid#189564PurposeCaHygB hygromycin resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicansDepositorInsertHygB
ExpressionYeastAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFS554
Plasmid#242854PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW3 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS553
Plasmid#242853PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW2 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS555
Plasmid#242855PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW4 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB_tetO7_sfGFP
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
ExpressionYeastPromotertetO7 promoterAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRExpressionYeastAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAPZa-STE2-1D4
Plasmid#133422PurposeMutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.DepositorAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only