8,418 results
-
Plasmid#20769DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
p416 25Q GAL
Plasmid#1185DepositorAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-ymScarletI-URA3
Plasmid#168055PurposeReplaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymScarletI
TagsymScarletIExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-mNeonGreen-KanMX6
Plasmid#129099PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
ExpressionYeastAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS159
Plasmid#122378PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGHYB
Plasmid#87447PurposePichia pastoris expression plasmidDepositorPublicationTypeEmpty backboneExpressionYeastPromoterGAPAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML104-NatMx3
Plasmid#83477PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.DepositorInsertNatMx3
ExpressionYeastAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorPublicationInsertYeast-optimized dCas9
TagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1
Plasmid#58435PurposeInduces Citrine expression from a promoter containing 8 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)8-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HO-pGAL-poly-KanMX4-HO
Plasmid#51664Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoterDepositorTypeEmpty backboneUseYeast integrationExpressionYeastAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pREP41-MCS+
Plasmid#52690PurposeSame as pREP41, with better MCSDepositorTypeEmpty backboneExpressionYeastPromoterPnmt41Available sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDZ513 (pHIS MET25pro PP7-PS-yeGFP)
Plasmid#45931DepositorInsertPCP-PS
Tags2x-yeGFPExpressionYeastPromoterMet25Available sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-Kan
Plasmid#44955PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selectionDepositorInsertyoTagRFP657
ExpressionYeastAvailable sinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-yoSuperfolderGFP-Kan
Plasmid#44901PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with Kan selectionDepositorInsertyoSuperfolderGFP
ExpressionYeastAvailable sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-link-yoSuperfolderGFP-CaURA3
Plasmid#44873PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with CaUra3 selectionDepositorInsertyoSuperfolderGFP
ExpressionYeastAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mCherry-SpHis5
Plasmid#44643PurposeYeast tagging vector to create gene fusion with mCherry with SpHis5 selectionDepositorInsertmCherry
ExpressionYeastAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDR196
Plasmid#36029DepositorTypeEmpty backboneExpressionYeastAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDS277
Plasmid#34973PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationV529NAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only