8,431 results

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pFA6a-link-yETSapphire-CaUra3

Plasmid

#44650

Depositor

Kurt Thorn

Insert

Turbo Sapphire

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pVS2

Plasmid

#33144

Depositor

Michael Rosbash

Publication

Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

Inserts

LacO (256 copies)
GAL-GFP-kd200-GALpA reporter

Expression

Yeast

Mutation

GFP is mutated such that amino acids 64-73 (FSYGV…

Promoter

bidirectional GAL1-GAL10 promoter

Available since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
pDR-GW FLII12Pglu-700μδ6

Plasmid

#28002

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLII12Pglu-700μδ6

Tags

eCFP and eYFP

Expression

Yeast

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Publication

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFA6a-6xGLY-FLAG-HIS3MX6

Plasmid

#20750

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFA6a-6xGLY-His-tag-HIS3MX6

Plasmid

#20762

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Publication

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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JME4580

Plasmid

#129659

Purpose

HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Publication

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-mNeonGreen-KanMX6

Plasmid

#129099

Purpose

For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

Depositor

Wei-Lih Lee

Insert

mNeonGreen

Expression

Yeast

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRS159

Plasmid

#122378

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Publication

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGHYB

Plasmid

#87447

Purpose

Pichia pastoris expression plasmid

Depositor

Sheng Yang

Publication

Yang et al Yeast. 2014 Apr;31(4):115-25.

Type

Empty backbone

Expression

Yeast

Promoter

GAP

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRISPRyl_A08

Plasmid

#84610

Purpose

Introduces DSB at A08 locus in Y. lipolytica

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDuRCC-N

Plasmid

#81194

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Publication

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please view depositor comments below and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTPGI_dCas9

Plasmid

#64279

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Tags

SV40 NLS

Expression

Yeast

Mutation

D10A & H840A relative to S. pyogenes Cas9

Promoter

pTPGI

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1

Plasmid

#58435

Purpose

Induces Citrine expression from a promoter containing 8 lexA boxes in yeast cells

Depositor

Joerg Stelling

Publication

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)8-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HO-pGAL-poly-KanMX4-HO

Plasmid

#51664

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

Depositor

David Stillman

Publication

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pREP41-MCS+

Plasmid

#52690

Purpose

Same as pREP41, with better MCS

Depositor

Michael Nick Boddy

Type

Empty backbone

Expression

Yeast

Promoter

Pnmt41

Available since

April 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDZ513 (pHIS MET25pro PP7-PS-yeGFP)

Plasmid

#45931

Depositor

Daniel Zenklusen

Publication

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

PCP-PS

Tags

2x-yeGFP

Expression

Yeast

Promoter

Met25

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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