8,173 results
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Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-DIPP1-HA
Plasmid#183946PurposeExpresses human HA-tagged DIPP1 from yeast GPD promoterDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416 103Q GAL
Plasmid#1186DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pNXRVa-HACre
Plasmid#78398Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
kanMX6
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
ExpressionYeastPromoterAOX1 promoterAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP-GM1
Plasmid#64739PurposeYeast expression plasmidDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-HIS3MX6
Plasmid#129100PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
ExpressionYeastAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0532
Plasmid#133502PurposeExpression of mTagBFP2 targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mTagBFP2-terminator(tdh1)-kanMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-HIS3MX6
Plasmid#20762DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-DuDre-Atg8-404
Plasmid#69201PurposeRFP-Atg8 (PAS marker) with improved propertyDepositorInsertDuDre-Atg8
TagsDsRed Express 2ExpressionYeastAvailable SinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
BS-Ura3-GFP-Atg8
Plasmid#69194PurposeSingle copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)DepositorInsertGFP-Atg8
TagsEGFPExpressionYeastAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterENO1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDZ276 (pURA MET28 PCP-2x-yeGFP)
Plasmid#35194DepositorInsertPCP-2x-yeGFP
ExpressionYeastAvailable SinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mYFP-hphMX6
Plasmid#105155PurposeYeast gene targetingDepositorInsertmYFP-hphMX6
TagsmYFPExpressionBacterial and YeastMutationA206KAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BYG
Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
Tags8xhistidineExpressionYeastPromoterTDH3Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p404-BrdU-Inc
Plasmid#71790PurposeBrdU-incorporating plasmid with selectable marker TRP1DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS2142
Plasmid#184273PurposeTet-off genome insertion systemDepositorInsertCatTA
ExpressionYeastPromoterTDH3Available SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-P525L-YFP
Plasmid#29628DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS424GFP-2xPH(Osh2)
Plasmid#36095DepositorInsert2xPH(Osh2) fusion
TagsGFPExpressionYeastPromoterPRC1Available SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWS3865
Plasmid#185742PurposeSub-array backbone 1/4DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ube2c
Plasmid#31418DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PAmCherry-KanMX6
Plasmid#87026PurposeTag S. pombe genes in C terminal with PAmCherryDepositorInsertPAmCherry
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXP422
Plasmid#26845DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSII42H-TDH3pr-mNeon
Plasmid#194539PurposeHigh copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeonDepositorInserthphMX
ExpressionYeastPromoterTDH3Available SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_GAL2
Plasmid#166088PurposePlasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeastDepositorAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL28-decoder
Plasmid#159450PurposeInsert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.DepositorInsertPlexAtet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPlexAtetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
TagsmClover3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZJOM153
Plasmid#133642PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ytdTomato
Plasmid#118456PurposeOverexpression of yeast codon-optimized tdTomatoDepositorInsertytdTomato
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXP418
Plasmid#26843DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-bleMX6
Plasmid#19344DepositorInsertbleMX6
ExpressionYeastAvailable SinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pROS17
Plasmid#107931PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-EGFP-ccdB
Plasmid#14314DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
FRP991_PACT1(-1-520)-LexA-ER-Gal4AD
Plasmid#58438PurposeExpresses LexA-ER-Gal4AD from yeast cellsDepositorInsertLexA-ER-Gal4AD
ExpressionYeastPromoterAct1 promoter (-520;1)Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-yomRuby3-SpHIS5
Plasmid#204287PurposeFor C-terminal tagging using yeast optimized mRuby3DepositorInsertpFA6-yomRuby3-SpHIS5
TagsyomRuby3ExpressionYeastPromoternoneAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_12_GlyRNA
Plasmid#177710PurposePlasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
TagsLifeActExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx2(yeast)
Plasmid#65005PurposeYeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2DepositorInsertGRX2
TagsroGFP2ExpressionYeastAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDM291-tetR-tup11Δ70
Plasmid#41027DepositorInserttetR-tup11Δ70
ExpressionYeastMutationtup11 starts at aa70Available SinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomTagBFP-Kan
Plasmid#44898PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP with Kan selectionDepositorInsertyomTagBFP
ExpressionYeastAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTEF6-7
Plasmid#124583PurposeReporter plasmid with constitutively expressed mCherry and YFP (eCitrine)DepositorInsertmCherry
ExpressionYeastAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-X0
Plasmid#115565PurposeControl vector expressing GFP alone without any consensus repeatsDepositorInsertGreen Fluorescent Protein
ExpressionBacterial and YeastPromoterADH1Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM20
Plasmid#133644PurposeIntegration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only