8,017 results

1. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

   Plasmid

   #178989

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAV0753

   Plasmid

   #133487

   Purpose

   Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. YEp195-CUP1-His-Smt3

   Plasmid

   #99538

   Purpose

   Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

   Depositor

   Helle Ulrich

   Article

   Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS426-GFP-Osh1-PH

   Plasmid

   #71372

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh1-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa267-388

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCggRO-reporter

   Plasmid

   #124582

   Purpose

   Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

   Depositor

   Matthias Heinemann

   Article

   Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

   Insert

   CggR promoter element
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

   Plasmid

   #173453

   Purpose

   Contains mCherry-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mCherry-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG416GAL-EMCV_3C

   Plasmid

   #201941

   Purpose

   Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   EMCV 3C protease (EMCVgp1 )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p426 25Q GPD

   Plasmid

   #1181

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pPTK020-7-attB

   Plasmid

   #84989

   Purpose

   Type 7, BxbI recognition site

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   BxbI recognition site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   BsaI site removed (1711 t to a)

   Promoter

   Non

   Available since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM09

    Plasmid

    #135479

    Purpose

    Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunHsp70pr
    

    Use

    Tags

    GFP and hph

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Spun Hsp70pr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUDP013

    Plasmid

    #103873

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV0327

    Plasmid

    #133488

    Purpose

    Expression of nucleo-cytoplasmic sfGFP

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. NM R2E2 GFP

    Plasmid

    #1208

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 NM domain (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    2 additional copies of second oligopeptide repeat…

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDDGFP-Leu2d-ScVrg4

    Plasmid

    #102334

    Purpose

    Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

    Insert

    YGL225W (VRG4 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYB-Dual(mNG(yeast-opt))

    Plasmid

    #216727

    Purpose

    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

    Depositor

    Emil Jensen

    Article

    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pGAL1(yeast) and pT7-RNAP(bacteria)

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pREF-GFP kan

    Plasmid

    #122061

    Purpose

    reporter plasmid for termination and terminator override to express GFP. Marked with URA3

    Depositor

    Daniel Reines

    Article

    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

    Inserts

    intergenic terminator from yeast IMD2
    green fluorescent protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL1 comes in pYES2

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWS481

    Plasmid

    #123029

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (6x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAL498

    Plasmid

    #169154

    Purpose

    Encodes OptoQ-INVRT4

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

    Plasmid

    #140153

    Purpose

    Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPICZA_CeTRIC-B1_Del

    Plasmid

    #87344

    Purpose

    Inducible expression of CeTRIC-B1 in P.Pichia strains

    Depositor

    Zhenfeng Liu

    Article

    Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

    Insert

    TRIC-B1
    

    Use

    Tags

    polyhistidine

    Expression

    Yeast

    Mutation

    Δ248-297

    Promoter

    AOX1

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ZJOM100

    Plasmid

    #133662

    Purpose

    Integration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mito-yHS1

    Plasmid

    #159153

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1
    

    Use

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pFA6a-GBP-hphMX6

    Plasmid

    #105159

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

    Insert

    GBP-hphMX6
    

    Use

    Tags

    GBP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

27. pYes2-TaPCS1

    Plasmid

    #49767

    Purpose

    Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

    Depositor

    Julian Schroeder

    Article

    Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

    Insert

    TaPCS1 (PCS1 Triticum aestivum)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pKL1-222

    Plasmid

    #219409

    Purpose

    Nuclear expression of Synrophin PDZ clamp protein

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    pTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDS240

    Plasmid

    #34966

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K533∆, high-affinity epitope

    Promoter

    GAL1

    Available since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXP741

    Plasmid

    #46059

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBBK67 pHomL-RPL18Bp-RPC19-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179051

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Scarlet(rfp), a marker for the nucleolus

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-RPC19-Link-Scarlet(rfp)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIM700

    Plasmid

    #74639

    Purpose

    alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRG710_lexO-HO_LexA-TF_HIS3MX

    Plasmid

    #154819

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pXP320

    Plasmid

    #26838

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG426-GAL-UBQLN2

    Plasmid

    #181708

    Purpose

    Galactose inducible expression of UBQLN2

    Depositor

    Alejandro Chavez

    Insert

    UBQLN2 (UBQLN2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS423-TyrSgH

    Plasmid

    #163973

    Purpose

    Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFA6a-12Pk-kanMX6

    Plasmid

    #49176

    Purpose

    genomic 12Pk epitope tagging in S. pombe

    Depositor

    Eishi Noguchi

    Article

    Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

    Type

    Empty backbone

    Use

    Tags

    12Pk

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pI2-MTU-DO-URA

    Plasmid

    #160015

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pI1-MTU-DO-LEU

    Plasmid

    #160013

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJW1234

    Plasmid

    #62367

    Purpose

    pRS305 pPGK1-BirA::LEU2

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA (birA )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge