Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 482 results for: Numb;

Clear keywords
Showing: 1 - 40 of 482 results

  8. PWPXLD-GFP-Numb-S276D

    Plasmid
    #133752
    Purpose
    Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD
    Depositor
    Chay Kuo
    Article
    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4
    Insert
    NUMB endocytic adaptor protein (Numb Mouse)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    serine 276 converted to aspartic acid
    Promoter
    EF1-alpha
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133752-plasmid-map-sequence-id-264923

    Enlarge

  10. NUMBLA

    Plasmid
    #42420
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUMBLA (NUMBL Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

    Enlarge

  16. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Rodolphe Barrangou, Todd Klaenhammer
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Expression
    Bacterial
    Available Since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

    Enlarge

  25. pET28a-SnoopCatcher

    Plasmid
    #72322
    Purpose
    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a-SnoopCatcher
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    changed G842T and D848G of S. pneumoniae adhesin …
    Promoter
    T7
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72322-plasmid-map-sequence-id-498732

    Enlarge

  33. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-487542

    Enlarge

  35. pAC-BETA

    Plasmid
    #53272
    Purpose
    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.
    Insert
    crtE, crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53272-plasmid-map-sequence-id-487543

    Enlarge

  38. pSP6-RNAP

    Plasmid
    #48143
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase SP6
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    V314A (numbering based on NCBI Sequence ID: NP_85…
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48143-plasmid-map-sequence-id-373044

    Enlarge

  39. FUGW-Arl13b-PR-EGFP-TurboID

    Plasmid
    #248194
    Purpose
    Lentiviral expression of truncated Arl13b containing the Proline-Rich domain at the C-terminus (cytosol localization)
    Depositor
    Xuecai Ge
    Article
    Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:
    Insert
    The Arl13b noncilium targeting sequence-EGFP-TurboID (Arl13b Mouse)
    Use
    Lentiviral
    Tags
    EGFP and TurboID
    Expression
    Mammalian
    Mutation
    Truncated to only contain the PR domain
    Promoter
    hUBC
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    248194-plasmid-map-sequence-id-494577

    Enlarge

  40. PWPXLD-EGFR-P667A

    Plasmid
    #133750
    Purpose
    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid
    Depositor
    Chay Kuo
    Article
    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4
    Insert
    Mutant P667A EGFR-HA (EGFR Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    proline 667 converted to alanine (Please see depo…
    Promoter
    EF1-alpha
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133750-plasmid-map-sequence-id-264885

    Enlarge

Showing: 1 - 40 of 482 results