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Showing: 1 - 40 of 43 results

  1. pET28a-mxEH

    Plasmid
    #104977
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Insert
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    104977-plasmid-map-sequence-id-202891

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  2. pT7-7 asyn 1-106AA Mxe Histag

    Plasmid
    #105745
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein 1-106AA Mxe Histag (SNCA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    1-106AA Mxe
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105745-plasmid-map-sequence-id-207300

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  3. pT7-7 asyn WT1-106 Mxe

    Plasmid
    #105746
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein 1-106 Mxe (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    1-106AA Mxe
    Promoter
    T7
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105746-plasmid-map-sequence-id-209704

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  4. pJCX4

    Plasmid
    #170756
    Purpose
    Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Use
    Tags
    Mxe GyrA intein - chitin binding domain (CBD)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170756-plasmid-map-sequence-id-334719

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  7. pTXB1-hMeCP2

    Plasmid
    #48091
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged
    Promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pTXB1-hMeCP2-R270X

    Plasmid
    #48092
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270X
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pTXB1-hMeCP2-G273X

    Plasmid
    #48093
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pTXB3-Flag PLK1

    Plasmid
    #68271
    Purpose
    bacterial expression of Plk1
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pTXB3-Flag PLK1 T210D

    Plasmid
    #68272
    Purpose
    bacterial expression of Plk1 T210D mutant
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    T210D
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

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  14. pTXBI-mclover-YTHDC1-FL

    Plasmid
    #177121
    Purpose
    Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177121-plasmid-map-sequence-id-348688

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  15. pTXBI-mclover-YTH-IDR2

    Plasmid
    #177122
    Purpose
    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH and IDR2 domains only (IDR1 deletion)
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177122-plasmid-map-sequence-id-348676

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  26. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Use
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  27. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Article
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Use
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

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  28. pTXB1-H2B(1-114)_wt

    Plasmid
    #118252
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118252-plasmid-map-sequence-id-226720

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  29. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  30. pTXB1-TP5

    Plasmid
    #160088
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160088-plasmid-map-sequence-id-313736

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  31. pTXB1-TP3

    Plasmid
    #160086
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160086-plasmid-map-sequence-id-313733

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  35. pTXB1-TP4

    Plasmid
    #160087
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160087-plasmid-map-sequence-id-313735

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  36. pTXB1-TP1

    Plasmid
    #160084
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein, no linker
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160084-plasmid-map-sequence-id-314364

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  37. pTXB1-TP2

    Plasmid
    #160085
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160085-plasmid-map-sequence-id-313737

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  38. pET30a-HS-Tn5

    Plasmid
    #127916
    Purpose
    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.
    Depositor
    Silin Zhong
    Article
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Hyperstable Tn5
    Use
    Tags
    E coli elongation factor Ts and Mxe intein - Chit…
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127916-plasmid-map-sequence-id-248754

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  39. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Use
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. pACYCduet-mxLOX1-EH

    Plasmid
    #104978
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Deokkun Oh
    Inserts
    MXAN_1745 (MXAN_1745 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7promoter
    Available since
    Feb. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    104978-plasmid-map-sequence-id-203225

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Showing: 1 - 40 of 43 results