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Showing: 451 - 500 of 1752 results

  2. pCMV-3UTR

    Plasmid
    #202550
    Purpose
    Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media
    Depositor
    Rostislav Skrabana
    Article
    Meskova et al Antibodies (Basel). 2023 Aug 4;12(3):51.
    Type
    Empty backbone
    Use
    Tags
    ATG initiation codon followed by an IGKV3 signal …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    202550-plasmid-map-sequence-id-405353

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  7. pLX304-EZH1-dPSET

    Plasmid
    #203586
    Purpose
    Expresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.
    Depositor
    William Kaelin, Abhishek Chakraborty
    Article
    Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5
    Insert
    EZH1 (EZH1 Human)
    Use
    Lentiviral
    Tags
    V5-tagged
    Expression
    Mammalian
    Mutation
    deletion of aa 744-747 and partial resistance to …
    Promoter
    CMV
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    203586-plasmid-map-sequence-id-423597

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  21. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Article
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Insert
    Histone H2B - Citrine (H2BC21 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    5xTetO pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. sUPRa

    Plasmid
    #223242
    Purpose
    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels
    Depositor
    Colin Akerman
    Article
    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10
    Insert
    sUPRa
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Custom
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    223242-plasmid-map-sequence-id-450772

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  26. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Promoter
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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  38. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  39. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  42. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid
    #201955
    Purpose
    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201955-plasmid-map-sequence-id-405309

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  49. pO5

    Plasmid
    #193749
    Purpose
    Expresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-
    Insert
    tdTomato
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PIAH
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193749-plasmid-map-sequence-id-390459

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  50. pO3

    Plasmid
    #193748
    Purpose
    Expresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-
    Insert
    E2-Crimson
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PAAH
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193748-plasmid-map-sequence-id-390452

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Showing: 451 - 500 of 1752 results