We narrowed to 1,583 results for: pbluescript

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EasyFusion T2A-H2B-GFP

Plasmid

#112851

Purpose

General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-GFP

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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PDX1-2A-eGFP

Plasmid

#66964

Purpose

PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

pancreatic and duodenal homeobox 1 (PDX1 Human)

Use

Unspecified

Tags

2A-eGFP

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-GGAC-ATGC

Plasmid

#60949

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-MXT GIRK2

Plasmid

#20675

Depositor

Henry Lester

Article

Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

Insert

Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)

Use

Xenopus expression

Tags

3' UTR Xenopus B- globin and 5' UTR Xen…

Mutation

A369V

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pBS IRES EGFP (DM#271)

Plasmid

#15080

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

IRES EGFP

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
tet H-ras val12

Plasmid

#8900

Depositor

Lynda Chin

Article

Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

Insert

ras val12 (HRAS Human)

Tags

Tet-op promoter

Expression

Mammalian

Mutation

G12V

Available Since

Jan. 13, 2006

Availability

Academic Institutions and Nonprofits only
3265 pBS Mcl-1

Plasmid

#8763

Depositor

Stanley Korsmeyer

Article

Rinkenberger et al Genes Dev 2000 Jan 1;14(1):23-7.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Cloning vector

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pLF3FShC

Plasmid

#153083

Purpose

RMCE integration vector with self excising cassette

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMK281 (mCherry2-Hygro)

Plasmid

#72797

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Hygro

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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p3xP3-EGFP.vas-int.NLS

Plasmid

#60948

Purpose

Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

vas-phiC31 integrase.NLS

Use

Rmce

Promoter

vasa

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only
Lox-stop-Lox p53 R270H

Plasmid

#14853

Depositor

Tyler Jacks

Article

Olive et al Cell. 2004 Dec 17. 119(6):847-60.

Insert

p53 R270H (Trp53 Mouse)

Use

Cre/Lox

Tags

Lox-STOP-lox

Expression

Mammalian

Mutation

Contact mutant R270H. (Murine p53 codon 270 corre…

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
EasyFusion T2A-H2B-mCherry

Plasmid

#113089

Purpose

General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-mCherry

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMK283 (Stag-3FLAG-Neo)

Plasmid

#72799

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Neo

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSDMA57

Plasmid

#67941

Purpose

Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains NEO resistance marker.

Depositor

James Fraser

Article

Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

Type

Empty backbone

Use

Cryptococcal complementation vector

Promoter

ACT1 for Cryptococcal marker

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pT2HLacZpA1I.7 (#59)

Plasmid

#35965

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter (Tek Mouse)
SDK LacZ
SV40 pA
Tie2 full enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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I-SceI pB 5UAS::ZfSyGCaMP2

Plasmid

#26125

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (sypb Zebrafish)

Use

Zebrafish expression

Tags

GCaMP2

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pUAS-GG

Plasmid

#24342

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Type

Empty backbone

Tags

5XGAL4

Expression

Mammalian

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-SK-QS

Plasmid

#24370

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QS

Expression

Bacterial

Available Since

April 2, 2010

Availability

Academic Institutions and Nonprofits only

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mFGF4

Plasmid

#22085

Depositor

Gail Martin

Article

Hebert et al Dev Biol. 1990 Apr . 138(2):454-63.

Insert

FGF4 (Fgf4 Mouse)

Use

Bacterial

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only